000128059 001__ 128059
000128059 005__ 20231108213503.0
000128059 0247_ $$2doi$$a10.3389/frfst.2023.1209452
000128059 0248_ $$2sideral$$a135186
000128059 037__ $$aART-2023-135186
000128059 041__ $$adeu
000128059 100__ $$0(orcid)0000-0003-4744-8649$$aDelso, Carlota$$uUniversidad de Zaragoza
000128059 245__ $$aSynergetic effect of combining PEF treatments with sublethal doses of SO2 on the inactivation of Saccharomyces bayanus and Brettanomyces bruxellensis in red wine
000128059 260__ $$c2023
000128059 5060_ $$aAccess copy available to the general public$$fUnrestricted
000128059 5203_ $$aCertain microorganisms are capable of proliferating in wine despite its low pH and high ethanol content. The yeasts of the Saccharomyces genus responsible for alcoholic fermentation can alter wines with residual sugars; the proliferation of Brettanomyces bruxellensis brings about thoroughly unpleasant sensory changes. The main strategy currently applied in wineries for microbial control is the addition of sulfites (SO2). However, sulfites are being researched due to the symptoms they can cause in allergic individuals. Pulsed electric field (PEF) technology has the capability of inactivating vegetative cells of microorganisms at non-lethal temperatures and could thus prove to be an alternative to SO2. In this study, the resistance of Saccharomyces bayanus and B. bruxellensis suspended in wine to a series of different PEF treatments (10–25 kV/cm; 25–1000 µs; 40–170 kJ/kg) combined with sublethal concentrations of SO2 (10, 25, and 50 ppm) was evaluated. The results showed that even the least intense PEF treatments (10 kV/cm; 115 kJ/kg) inactivated more than 4.0 Log10 cycles in both types of yeasts immediately after treatment. The subsequent incubation of the treated yeasts for 24 h in wine managed to increase inactivation by 3.0 Log10 cycles. The combination of a moderate PEF treatment with sublethal doses of SO2 had a synergistic lethal effect on the two yeasts under study after 24 h of incubation in wine, leading to counts lying below the detection limit (>5.0 Log10 cycles). This synergistic effect was attributed to the existence of a portion of the population that had been sublethally damaged by PEF and in which SO2 could more easily penetrate the cytoplasm. These results demonstrate the capacity of PEF technology for microbial control of spoilage yeasts in wine. PEF could thus represent an alternative with the potential of eliminating or reducing SO2 levels in the winemaking process.
000128059 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000128059 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000128059 700__ $$aBerzosa, Alejandro$$uUniversidad de Zaragoza
000128059 700__ $$aSanz, Jorge$$uUniversidad de Zaragoza
000128059 700__ $$0(orcid)0000-0003-2430-858X$$aÁlvarez, Ignacio$$uUniversidad de Zaragoza
000128059 700__ $$0(orcid)0000-0003-3957-9091$$aRaso, Javier$$uUniversidad de Zaragoza
000128059 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000128059 773__ $$g3 (2023), 1209452 [10 pp.]$$pFront. food sci. technol. (Lausanne)$$tFrontiers in Food Science and Technology$$x2674-1121
000128059 8564_ $$s1314740$$uhttps://zaguan.unizar.es/record/128059/files/texto_completo.pdf$$yVersión publicada
000128059 8564_ $$s2008587$$uhttps://zaguan.unizar.es/record/128059/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000128059 909CO $$ooai:zaguan.unizar.es:128059$$particulos$$pdriver
000128059 951__ $$a2023-11-08-20:02:50
000128059 980__ $$aARTICLE