128099 20231108213504.0 doi 10.1016/j.celrep.2021.108757 sideral 135224 ART-2021-135224 eng Layunta, E. Universidad de Zaragoza (orcid)0000-0001-5573-6144 IL-22 promotes the formation of a MUC17 glycocalyx barrier in the postnatal small intestine during weaning 2021 The intestine is under constant exposure to chemicals, antigens, and microorganisms from the external environment. Apical aspects of transporting epithelial cells (enterocytes) form a brush-border membrane (BBM), shaped by packed microvilli coated with a dense glycocalyx. We present evidence showing that the glycocalyx forms an epithelial barrier that prevents exogenous molecules and live bacteria from gaining access to BBM. We use a multi-omics approach to investigate the function and regulation of membrane mucins exposed on the BBM during postnatal development of the mouse small intestine. Muc17 is identified as a major membrane mucin in the glycocalyx that is specifically upregulated by IL-22 as part of an epithelial defense repertoire during weaning. High levels of IL-22 at time of weaning reprogram neonatal postmitotic progenitor enterocytes to differentiate into Muc17-expressing enterocytes, as found in the adult intestine during homeostasis. Our findings propose a role for Muc17 in epithelial barrier function in the small intestine. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 9.995 2021 CELL BIOLOGY 33 / 195 = 0.169 2021 Q1 T1 4.845 2021 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2021 Q1 15.1 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Jäverfelt, S. Dolan, B. Arike, L. Pelaseyed, T. 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 34, 7 (2021), 108757 [24 pp.] Cell Reports Cell Reports 2211-1247 9794696 http://zaguan.unizar.es/record/128099/files/texto_completo.pdf Versión publicada 1353691 http://zaguan.unizar.es/record/128099/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:128099 articulos driver 2023-11-08-20:04:15 ARTICLE