000128133 001__ 128133
000128133 005__ 20241125101145.0
000128133 0247_ $$2doi$$a10.3389/fvets.2023.1176772
000128133 0248_ $$2sideral$$a135282
000128133 037__ $$aART-2023-135282
000128133 041__ $$aeng
000128133 100__ $$0(orcid)0000-0001-9818-508X$$aBarrachina, Laura$$uUniversidad de Zaragoza
000128133 245__ $$aInduced pluripotent stem cells in companion animals: how can we move the field forward?
000128133 260__ $$c2023
000128133 5060_ $$aAccess copy available to the general public$$fUnrestricted
000128133 5203_ $$aFollowing a one medicine approach, the development of regenerative therapies for human patients leads to innovative treatments for animals, while pre-clinical studies on animals provide knowledge to advance human medicine. Among many different biological products under investigation, stem cells are among the most prominent. Mesenchymal stromal cells (MSCs) are extensively investigated, but they present challenges such as senescence and limited differentiation ability. Embryonic stem cells (ESCs) are pluripotent cells with a virtually unlimited capacity for self-renewal and differentiation, but the use of embryos carries ethical concerns. Induced pluripotent stem cells (iPSCs) can overcome all of these limitations, as they closely resemble ESCs but are derived from adult cells by reprogramming in the laboratory using pluripotency-associated transcription factors. iPSCs hold great potential for applications in therapy, disease modeling, drug screening, and even species preservation strategies. However, iPSC technology is less developed in veterinary species compared to human. This review attempts to address the specific challenges associated with generating and applying iPSCs from companion animals. Firstly, we discuss strategies for the preparation of iPSCs in veterinary species and secondly, we address the potential for different applications of iPSCs in companion animals. Our aim is to provide an overview on the state of the art of iPSCs in companion animals, focusing on equine, canine, and feline species, as well as to identify which aspects need further optimization and, where possible, to provide guidance on future advancements. Following a “step-by-step” approach, we cover the generation of iPSCs in companion animals from the selection of somatic cells and the reprogramming strategies, to the expansion and characterization of iPSCs. Subsequently, we revise the current applications of iPSCs in companion animals, identify the main hurdles, and propose future paths to move the field forward. Transferring the knowledge gained from human iPSCs can increase our understanding in the biology of pluripotent cells in animals, but it is critical to further investigate the differences among species to develop specific approaches for animal iPSCs. This is key for significantly advancing iPSC application in veterinary medicine, which at the same time will also allow gaining pre-clinical knowledge transferable to human medicine.
000128133 536__ $$9info:eu-repo/grantAgreement/EC/H2020/101026825/EU/Cartilage derived from equine induced pluripotent stem cells: an in vitro and ex vivo One Medicine approach for osteoarthritis/CAREQiPSC$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101026825-CAREQiPSC
000128133 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000128133 590__ $$a2.6$$b2023
000128133 592__ $$a0.734$$b2023
000128133 591__ $$aVETERINARY SCIENCES$$b22 / 167 = 0.132$$c2023$$dQ1$$eT1
000128133 593__ $$aVeterinary (miscellaneous)$$c2023$$dQ1
000128133 594__ $$a4.8$$b2023
000128133 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000128133 700__ $$aArshaghi Eslami, Tarlan
000128133 700__ $$aO'Brien, Aisling
000128133 700__ $$aIvanovska, Ana
000128133 700__ $$aBarry, Frank
000128133 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000128133 773__ $$g10 (2023), 1176772 [18 pp.]$$pFront. vet. sci.$$tFrontiers in Veterinary Science$$x2297-1769
000128133 8564_ $$s2136460$$uhttps://zaguan.unizar.es/record/128133/files/texto_completo.pdf$$yVersión publicada
000128133 8564_ $$s2135091$$uhttps://zaguan.unizar.es/record/128133/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000128133 909CO $$ooai:zaguan.unizar.es:128133$$particulos$$pdriver
000128133 951__ $$a2024-11-22-12:04:08
000128133 980__ $$aARTICLE