doi:10.1016/j.ifset.2023.103478engDelso, CarlotaOspina, SebastiánBerzosa, AlejandroRaso, JavierÁlvarez-Lanzarote, IgnacioDefining winery processing conditions for the decontamination of must and wine spoilage microbiota by Pulsed Electric Fields (PEF)ART-2023-135358This study investigated the PEF-resistance of Saccharomyces bayanus, Brettanomyces bruxellensis, Lactobacillus plantarum, and Oenococus oeni in must or wine under continuous PEF processing. Results showed the capacity of PEF to achieve 3.0-log10-cycles (CFU/mL) of inactivation of all the microorganisms under moderate conditions (< 155 kJ/kg). Developed tertiary models accurately predicted the effect of PEF parameters on microbial inactivation, and Monte Carlo simulation considered the variability of factors and the maximum assumable microbial load in the final treated product. Results showed that PEF-treatments at 15 kV/cm and 129 or 153 kJ/kg would ensure the adequate decontamination (< 10 CFU/mL) of spoilage microorganism in must or wine, respectively. Industrial relevance: PEF technology has been shown to achieve adequate levels of microbial inactivation (3-log10) in must and wine under industrial applicable processing parameters, making it a suitable alternative to SO2 or sterilizing filtration for microbial control in winemaking. Reductions of 3-log10 CFU/mL of must and wine microbiota were found by continuous flow PEF-processing at 15 to 25 kV/cm and 175 to 148 kJ/kg, parameters applicable at industrial scale at 1 ton/h.2023http://zaguan.unizar.es/record/12819610.1016/j.ifset.2023.103478http://zaguan.unizar.es/record/128196oai:zaguan.unizar.es:128196info:eu-repo/grantAgreement/ES/DGA/A03-23Rinfo:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalentThis project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801586-IberusTalentINNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES 89 (2023), 103478 [12 pp.]byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess