000129308 001__ 129308 000129308 005__ 20240104111804.0 000129308 0247_ $$2doi$$a10.1016/j.tvjl.2017.04.005 000129308 0248_ $$2sideral$$a101051 000129308 037__ $$aART-2017-101051 000129308 041__ $$aeng 000129308 100__ $$0(orcid)0000-0001-7188-0461$$aRomero, A.$$uUniversidad de Zaragoza 000129308 245__ $$aComparison of autologous bone marrow and adipose tissue derived mesenchymal stem cells, and platelet rich plasma, for treating surgically induced lesions of the equine superficial digital flexor tendon 000129308 260__ $$c2017 000129308 5060_ $$aAccess copy available to the general public$$fUnrestricted 000129308 5203_ $$aSeveral therapies have been investigated for equine tendinopathies, but satisfactory long term results have not been achieved consistently and a better understanding of the healing mechanism elicited by regenerative therapies is needed. The aim of this study was to assess the separate effects of autologous bone marrow (BM) and adipose tissue (AT) derived mesenchymal stem cells (MSCs), and platelet rich plasma (PRP), for treating lesions induced in the superficial digital flexor tendon (SDFT) of horses. Lesions were created surgically in both SDFTs of the forelimbs of 12 horses and were treated with BM-MSCs (six tendons), AT-MSCs (six tendons) or PRP (six tendons). The remaining six tendons received lactated Ringer''s solution as control. Serial ultrasound assessment was performed prior to treatment and at 2, 6, 10, 20 and 45 weeks post-treatment. At 45 weeks, histopathology and gene expression analyses were performed. At week 6, the ultrasound echogenicity score in tendons treated with BM-MSCs suggested earlier improvement, whilst all treatment groups reached the same level at week 10, which was superior to the control group. Collagen orientation scores on histological examination suggested a better outcome in treated tendons. Gene expression was indicative of better tissue regeneration after all treatments, especially for BM-MSCs, as suggested by upregulation of collagen type I, decorin, tenascin and matrix metalloproteinase III mRNA. Considering all findings, a clear beneficial effect was elicited by all treatments compared with the control group. Although differences between treatments were relatively small, BM-MSCs resulted in a better outcome than PRP and AT-MSCs. 000129308 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2008-02428 000129308 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000129308 590__ $$a1.773$$b2017 000129308 591__ $$aVETERINARY SCIENCES$$b26 / 139 = 0.187$$c2017$$dQ1$$eT1 000129308 592__ $$a0.979$$b2017 000129308 593__ $$aVeterinary (miscellaneous)$$c2017$$dQ1 000129308 593__ $$aAnimal Science and Zoology$$c2017$$dQ1 000129308 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000129308 700__ $$0(orcid)0000-0001-9818-508X$$aBarrachina, L.$$uUniversidad de Zaragoza 000129308 700__ $$0(orcid)0000-0002-4495-8857$$aRanera, B. 000129308 700__ $$0(orcid)0000-0002-1075-8267$$aRemacha, A.R.$$uUniversidad de Zaragoza 000129308 700__ $$0(orcid)0000-0001-6629-6842$$aMoreno, B.$$uUniversidad de Zaragoza 000129308 700__ $$0(orcid)0000-0002-1204-4356$$ade Blas, I.$$uUniversidad de Zaragoza 000129308 700__ $$0(orcid)0000-0001-6489-8399$$aSanz, A.$$uUniversidad de Zaragoza 000129308 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, F.J.$$uUniversidad de Zaragoza 000129308 700__ $$0(orcid)0000-0003-1286-4968$$aVitoria, A.$$uUniversidad de Zaragoza 000129308 700__ $$0(orcid)0000-0002-9951-1075$$aJunquera, C.$$uUniversidad de Zaragoza 000129308 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, P.$$uUniversidad de Zaragoza 000129308 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar, C.$$uUniversidad de Zaragoza 000129308 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética 000129308 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000129308 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología 000129308 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000129308 773__ $$g224 (2017), 76-84$$pVet. j.$$tVeterinary Journal$$x1090-0233 000129308 8564_ $$s896685$$uhttps://zaguan.unizar.es/record/129308/files/texto_completo.pdf$$yPostprint 000129308 8564_ $$s939589$$uhttps://zaguan.unizar.es/record/129308/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000129308 909CO $$ooai:zaguan.unizar.es:129308$$particulos$$pdriver 000129308 951__ $$a2024-01-04-10:59:36 000129308 980__ $$aARTICLE