129308 20240104111804.0 doi 10.1016/j.tvjl.2017.04.005 sideral 101051 ART-2017-101051 eng Romero, A. Universidad de Zaragoza (orcid)0000-0001-7188-0461 Comparison of autologous bone marrow and adipose tissue derived mesenchymal stem cells, and platelet rich plasma, for treating surgically induced lesions of the equine superficial digital flexor tendon 2017 Access copy available to the general public Unrestricted Several therapies have been investigated for equine tendinopathies, but satisfactory long term results have not been achieved consistently and a better understanding of the healing mechanism elicited by regenerative therapies is needed. The aim of this study was to assess the separate effects of autologous bone marrow (BM) and adipose tissue (AT) derived mesenchymal stem cells (MSCs), and platelet rich plasma (PRP), for treating lesions induced in the superficial digital flexor tendon (SDFT) of horses. Lesions were created surgically in both SDFTs of the forelimbs of 12 horses and were treated with BM-MSCs (six tendons), AT-MSCs (six tendons) or PRP (six tendons). The remaining six tendons received lactated Ringer''s solution as control. Serial ultrasound assessment was performed prior to treatment and at 2, 6, 10, 20 and 45 weeks post-treatment. At 45 weeks, histopathology and gene expression analyses were performed. At week 6, the ultrasound echogenicity score in tendons treated with BM-MSCs suggested earlier improvement, whilst all treatment groups reached the same level at week 10, which was superior to the control group. Collagen orientation scores on histological examination suggested a better outcome in treated tendons. Gene expression was indicative of better tissue regeneration after all treatments, especially for BM-MSCs, as suggested by upregulation of collagen type I, decorin, tenascin and matrix metalloproteinase III mRNA. Considering all findings, a clear beneficial effect was elicited by all treatments compared with the control group. Although differences between treatments were relatively small, BM-MSCs resulted in a better outcome than PRP and AT-MSCs. info:eu-repo/grantAgreement/ES/MINECO/AGL2008-02428 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 1.773 2017 VETERINARY SCIENCES 26 / 139 = 0.187 2017 Q1 T1 0.979 2017 Veterinary (miscellaneous) 2017 Q1 Animal Science and Zoology 2017 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Barrachina, L. Universidad de Zaragoza (orcid)0000-0001-9818-508X Ranera, B. (orcid)0000-0002-4495-8857 Remacha, A.R. Universidad de Zaragoza (orcid)0000-0002-1075-8267 Moreno, B. Universidad de Zaragoza (orcid)0000-0001-6629-6842 de Blas, I. Universidad de Zaragoza (orcid)0000-0002-1204-4356 Sanz, A. Universidad de Zaragoza (orcid)0000-0001-6489-8399 Vázquez, F.J. Universidad de Zaragoza (orcid)0000-0002-8712-2275 Vitoria, A. Universidad de Zaragoza (orcid)0000-0003-1286-4968 Junquera, C. Universidad de Zaragoza (orcid)0000-0002-9951-1075 Zaragoza, P. Universidad de Zaragoza (orcid)0000-0001-5740-0185 Rodellar, C. Universidad de Zaragoza (orcid)0000-0003-3289-2675 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 224 (2017), 76-84 Vet. j. Veterinary Journal 1090-0233 896685 http://zaguan.unizar.es/record/129308/files/texto_completo.pdf Postprint 939589 http://zaguan.unizar.es/record/129308/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:129308 articulos driver 2024-01-04-10:59:36 ARTICLE