000129309 001__ 129309 000129309 005__ 20240104111805.0 000129309 0247_ $$2doi$$a10.1007/s12035-017-0713-1 000129309 0248_ $$2sideral$$a101474 000129309 037__ $$aART-2017-101474 000129309 041__ $$aeng 000129309 100__ $$0(orcid)0000-0001-6199-2953$$aLarrodé, Pilar$$uUniversidad de Zaragoza 000129309 245__ $$aDREAM-dependent activation of astrocytes in amyotrophic lateral sclerosis 000129309 260__ $$c2017 000129309 5060_ $$aAccess copy available to the general public$$fUnrestricted 000129309 5203_ $$aAmyotrophic lateral sclerosis (ALS) is a neurodegenerative disease of unknown origin and characterized by a relentless loss of motor neurons that causes a progressive muscle weakness until death. Among the several pathogenic mechanisms that have been related to ALS, a dysregulation of calcium-buffering proteins in motor neurons of the brain and spinal cord can make these neurons more vulnerable to disease progression. Downstream regulatory element antagonist modulator (DREAM) is a neuronal calcium-binding protein that plays multiple roles in the nucleus and cytosol. The main aim of this study was focused on the characterization of DREAM and glial fibrillary acid protein (GFAP) in the brain and spinal cord tissues from transgenic SOD1G93A mice and ALS patients to unravel its potential role under neurodegenerative conditions. The DREAM and GFAP levels in the spinal cord and different brain areas from transgenic SOD1G93A mice and ALS patients were analyzed by Western blot and immunohistochemistry. Our findings suggest that the calcium-dependent excitotoxicity progressively enhanced in the CNS in ALS could modulate the multifunctional nature of DREAM, strengthening its apoptotic way of action in both motor neurons and astrocytes, which could act as an additional factor to increase neuronal damage. The direct crosstalk between astrocytes and motor neurons can become vulnerable under neurodegenerative conditions, and DREAM could act as an additional switch to enhance motor neuron loss. Together, these findings could pave the way to further study the molecular targets of DREAM to find novel therapeutic strategies to fight ALS. 000129309 536__ $$9info:eu-repo/grantAgreement/ES/FIS/PI14-00947 000129309 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000129309 590__ $$a5.076$$b2017 000129309 591__ $$aNEUROSCIENCES$$b44 / 261 = 0.169$$c2017$$dQ1$$eT1 000129309 592__ $$a1.614$$b2017 000129309 593__ $$aNeurology$$c2017$$dQ1 000129309 593__ $$aNeuroscience (miscellaneous)$$c2017$$dQ1 000129309 593__ $$aCellular and Molecular Neuroscience$$c2017$$dQ2 000129309 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000129309 700__ $$0(orcid)0000-0001-5193-7782$$aCalvo, Ana Cristina$$uUniversidad de Zaragoza 000129309 700__ $$0(orcid)0000-0002-7277-4318$$aMoreno-Martínez, Laura$$uUniversidad de Zaragoza 000129309 700__ $$0(orcid)0000-0002-7955-7164$$aDe la Torre, Miriam$$uUniversidad de Zaragoza 000129309 700__ $$0(orcid)0000-0003-2436-7205$$aMoreno-García, Leticia 000129309 700__ $$aMolina, Nora 000129309 700__ $$0(orcid)0000-0001-7453-2470$$aCastiella, Tomás$$uUniversidad de Zaragoza 000129309 700__ $$0(orcid)0000-0002-2711-066X$$aIñiguez, Cristina$$uUniversidad de Zaragoza 000129309 700__ $$0(orcid)0000-0003-4427-505X$$aPascual, Luis Fernando$$uUniversidad de Zaragoza 000129309 700__ $$0(orcid)0000-0001-5981-5448$$aMiana Mena, Francisco Javier$$uUniversidad de Zaragoza 000129309 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza 000129309 700__ $$aRamón y Cajal, Santiago 000129309 700__ $$0(orcid)0000-0001-5687-6704$$aOsta, Rosario$$uUniversidad de Zaragoza 000129309 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética 000129309 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología 000129309 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina 000129309 7102_ $$11000$$2020$$aUniversidad de Zaragoza$$bDpto. Anat.Pat.Med.Leg.For.To.$$cArea Anatomía Patológica 000129309 773__ $$g55, 1 (2017), 1-12$$pMol. neurobiol.$$tMOLECULAR NEUROBIOLOGY$$x0893-7648 000129309 8564_ $$s1639216$$uhttps://zaguan.unizar.es/record/129309/files/texto_completo.pdf$$yPostprint 000129309 8564_ $$s2471569$$uhttps://zaguan.unizar.es/record/129309/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000129309 909CO $$ooai:zaguan.unizar.es:129309$$particulos$$pdriver 000129309 951__ $$a2024-01-04-10:59:46 000129309 980__ $$aARTICLE