129309 20240104111805.0 doi 10.1007/s12035-017-0713-1 sideral 101474 ART-2017-101474 eng Larrodé, Pilar Universidad de Zaragoza (orcid)0000-0001-6199-2953 DREAM-dependent activation of astrocytes in amyotrophic lateral sclerosis 2017 Access copy available to the general public Unrestricted Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease of unknown origin and characterized by a relentless loss of motor neurons that causes a progressive muscle weakness until death. Among the several pathogenic mechanisms that have been related to ALS, a dysregulation of calcium-buffering proteins in motor neurons of the brain and spinal cord can make these neurons more vulnerable to disease progression. Downstream regulatory element antagonist modulator (DREAM) is a neuronal calcium-binding protein that plays multiple roles in the nucleus and cytosol. The main aim of this study was focused on the characterization of DREAM and glial fibrillary acid protein (GFAP) in the brain and spinal cord tissues from transgenic SOD1G93A mice and ALS patients to unravel its potential role under neurodegenerative conditions. The DREAM and GFAP levels in the spinal cord and different brain areas from transgenic SOD1G93A mice and ALS patients were analyzed by Western blot and immunohistochemistry. Our findings suggest that the calcium-dependent excitotoxicity progressively enhanced in the CNS in ALS could modulate the multifunctional nature of DREAM, strengthening its apoptotic way of action in both motor neurons and astrocytes, which could act as an additional factor to increase neuronal damage. The direct crosstalk between astrocytes and motor neurons can become vulnerable under neurodegenerative conditions, and DREAM could act as an additional switch to enhance motor neuron loss. Together, these findings could pave the way to further study the molecular targets of DREAM to find novel therapeutic strategies to fight ALS. info:eu-repo/grantAgreement/ES/FIS/PI14-00947 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 5.076 2017 NEUROSCIENCES 44 / 261 = 0.169 2017 Q1 T1 1.614 2017 Neurology 2017 Q1 Neuroscience (miscellaneous) 2017 Q1 Cellular and Molecular Neuroscience 2017 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Calvo, Ana Cristina Universidad de Zaragoza (orcid)0000-0001-5193-7782 Moreno-Martínez, Laura Universidad de Zaragoza (orcid)0000-0002-7277-4318 De la Torre, Miriam Universidad de Zaragoza (orcid)0000-0002-7955-7164 Moreno-García, Leticia (orcid)0000-0003-2436-7205 Molina, Nora Castiella, Tomás Universidad de Zaragoza (orcid)0000-0001-7453-2470 Iñiguez, Cristina Universidad de Zaragoza (orcid)0000-0002-2711-066X Pascual, Luis Fernando Universidad de Zaragoza (orcid)0000-0003-4427-505X Miana Mena, Francisco Javier Universidad de Zaragoza (orcid)0000-0001-5981-5448 Zaragoza, Pilar Universidad de Zaragoza (orcid)0000-0001-5740-0185 Ramón y Cajal, Santiago Osta, Rosario Universidad de Zaragoza (orcid)0000-0001-5687-6704 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 1000 020 Universidad de Zaragoza Dpto. Anat.Pat.Med.Leg.For.To. Area Anatomía Patológica 55, 1 (2017), 1-12 Mol. neurobiol. MOLECULAR NEUROBIOLOGY 0893-7648 1639216 http://zaguan.unizar.es/record/129309/files/texto_completo.pdf Postprint 2471569 http://zaguan.unizar.es/record/129309/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:129309 articulos driver 2024-01-04-10:59:46 ARTICLE