000129312 001__ 129312
000129312 005__ 20240104111805.0
000129312 0247_ $$2doi$$a10.1099/jgv.0.000685
000129312 0248_ $$2sideral$$a97667
000129312 037__ $$aART-2017-97667
000129312 041__ $$aeng
000129312 100__ $$0(orcid)0000-0002-5228-248X$$aSanz Rubio, D
000129312 245__ $$aIncreased circulating microRNAs miR-342-3p and miR-21-5p in natural sheep prion disease
000129312 260__ $$c2017
000129312 5060_ $$aAccess copy available to the general public$$fUnrestricted
000129312 5203_ $$aScrapie is a transmissible spongiform encephalopathy (TSE), or prion disease, of sheep and goats. As no simple diagnostic tests are yet available to detect TSEs in vivo, easily accessible biomarkers could facilitate the eradication of scrapie agents from the food chain. To this end, we analysed by quantitative reverse transcription PCR a selected set of candidate microRNAs (miRNAs) from circulating blood plasma of naturally infected, classical scrapie sheep that demonstrated clear scrapie symptoms and pathology. Significant scrapie-associated increase was repeatedly found for miR-342-3p and miR-21-5p. This is the first demonstration, to our knowledge, of circulating miRNA alterations in any animal suffering from TSE. Genome-wide expression studies are warranted to investigate the true depth of miRNA alterations in naturally occurring TSEs, especially in presymptomatic animals, as the presented study demonstrates the potential feasibility of miRNAs as circulating TSE biomarkers.
000129312 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A17$$9info:eu-repo/grantAgreement/ES/UZ/UZ2014-BIO-5
000129312 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000129312 590__ $$a2.514$$b2017
000129312 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b66 / 159 = 0.415$$c2017$$dQ2$$eT2
000129312 591__ $$aVIROLOGY$$b18 / 35 = 0.514$$c2017$$dQ3$$eT2
000129312 592__ $$a1.325$$b2017
000129312 593__ $$aVirology$$c2017$$dQ2
000129312 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000129312 700__ $$0(orcid)0000-0003-2454-2114$$aLópez-Pérez, O$$uUniversidad de Zaragoza
000129312 700__ $$ade Andrés Pablo, A
000129312 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, R$$uUniversidad de Zaragoza
000129312 700__ $$0(orcid)0000-0001-5687-6704$$aOsta, R$$uUniversidad de Zaragoza
000129312 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, JJ$$uUniversidad de Zaragoza
000129312 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, P$$uUniversidad de Zaragoza
000129312 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, I$$uUniversidad de Zaragoza
000129312 700__ $$0(orcid)0000-0002-7243-1737$$aToivonen, JM.$$uUniversidad de Zaragoza
000129312 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000129312 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000129312 773__ $$g98 (2017), 305-310$$pJ. gen. virol.$$tJOURNAL OF GENERAL VIROLOGY$$x0022-1317
000129312 8564_ $$s1719719$$uhttps://zaguan.unizar.es/record/129312/files/texto_completo.pdf$$yVersión publicada
000129312 8564_ $$s2769414$$uhttps://zaguan.unizar.es/record/129312/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000129312 909CO $$ooai:zaguan.unizar.es:129312$$particulos$$pdriver
000129312 951__ $$a2024-01-04-10:59:51
000129312 980__ $$aARTICLE