129312 20240104111805.0 doi 10.1099/jgv.0.000685 sideral 97667 ART-2017-97667 eng Sanz Rubio, D (orcid)0000-0002-5228-248X Increased circulating microRNAs miR-342-3p and miR-21-5p in natural sheep prion disease 2017 Access copy available to the general public Unrestricted Scrapie is a transmissible spongiform encephalopathy (TSE), or prion disease, of sheep and goats. As no simple diagnostic tests are yet available to detect TSEs in vivo, easily accessible biomarkers could facilitate the eradication of scrapie agents from the food chain. To this end, we analysed by quantitative reverse transcription PCR a selected set of candidate microRNAs (miRNAs) from circulating blood plasma of naturally infected, classical scrapie sheep that demonstrated clear scrapie symptoms and pathology. Significant scrapie-associated increase was repeatedly found for miR-342-3p and miR-21-5p. This is the first demonstration, to our knowledge, of circulating miRNA alterations in any animal suffering from TSE. Genome-wide expression studies are warranted to investigate the true depth of miRNA alterations in naturally occurring TSEs, especially in presymptomatic animals, as the presented study demonstrates the potential feasibility of miRNAs as circulating TSE biomarkers. info:eu-repo/grantAgreement/ES/DGA/A17 info:eu-repo/grantAgreement/ES/UZ/UZ2014-BIO-5 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.514 2017 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 66 / 159 = 0.415 2017 Q2 T2 VIROLOGY 18 / 35 = 0.514 2017 Q3 T2 1.325 2017 Virology 2017 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion López-Pérez, O Universidad de Zaragoza (orcid)0000-0003-2454-2114 de Andrés Pablo, A Bolea, R Universidad de Zaragoza (orcid)0000-0002-2746-3932 Osta, R Universidad de Zaragoza (orcid)0000-0001-5687-6704 Badiola, JJ Universidad de Zaragoza (orcid)0000-0002-7173-7216 Zaragoza, P Universidad de Zaragoza (orcid)0000-0001-5740-0185 Martín-Burriel, I Universidad de Zaragoza (orcid)0000-0001-6016-4726 Toivonen, JM. Universidad de Zaragoza (orcid)0000-0002-7243-1737 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 98 (2017), 305-310 J. gen. virol. JOURNAL OF GENERAL VIROLOGY 0022-1317 1719719 http://zaguan.unizar.es/record/129312/files/texto_completo.pdf Versión publicada 2769414 http://zaguan.unizar.es/record/129312/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:129312 articulos driver 2024-01-04-10:59:51 ARTICLE