129346 20240319081010.0 doi 10.1016/j.cma.2022.115477 sideral 130091 ART-2022-130091 eng Aparicio-Yuste, R. Universidad de Zaragoza (orcid)0000-0003-4617-8130 Computational modelling of epithelial cell monolayers during infection with Listeria monocytogenes 2022 Access copy available to the general public Unrestricted Intracellular bacterial infections alter the normal functionality of human host cells and tissues. Infection can also modify the mechanical properties of host cells, altering the mechanical equilibrium of tissues. In order to advance our understanding of host–pathogen interactions, simplified in vitro models are normally used. However, in vitro studies present certain limitations that can be alleviated by the use of computer-based models. As complementary tools these computational models, in conjunction with in vitro experiments, can enhance our understanding of the mechanisms of action underlying infection processes. In this work, we extend our previous computer-based model to simulate infection of epithelial cells with the intracellular bacterial pathogen Listeria monocytogenes. We found that forces generated by host cells play a regulatory role in the mechanobiological response to infection. After infection, in silico cells alter their mechanical properties in order to achieve a new mechanical equilibrium. The model pointed the key role of cell–cell and cell–extracellular matrix interactions in the mechanical competition of bacterial infection. The obtained results provide a more detailed description of cell and tissue responses to infection, and could help inform future studies focused on controlling bacterial dissemination and the outcome of infection processes. © 2022 The Author(s) info:eu-repo/grantAgreement/ES/MCIU/FPU20-05274 info:eu-repo/grantAgreement/ES/MICINN/PID2021-124271OB-I00 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 7.2 2022 MECHANICS 6 / 137 = 0.044 2022 Q1 T1 MATHEMATICS, INTERDISCIPLINARY APPLICATIONS 4 / 107 = 0.037 2022 Q1 T1 ENGINEERING, MULTIDISCIPLINARY 7 / 90 = 0.078 2022 Q1 T1 2.218 2022 Computational Mechanics 2022 Q1 Computer Science Applications 2022 Q1 Physics and Astronomy (miscellaneous) 2022 Q1 Mechanics of Materials 2022 Q1 Mechanical Engineering 2022 Q1 11.6 2022 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Serrano-Alcalde, F. Muenkel, M. Garcia-Aznar, J. Universidad de Zaragoza (orcid)0000-0002-9864-7683 Bastounis, E. E. Gomez-Benito, M. Universidad de Zaragoza (orcid)0000-0002-1878-8997 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 401, Part A (2022), 115477 [20 pp] Comput. methods appl. mech. eng. Computer Methods in Applied Mechanics and Engineering 0045-7825 3876759 http://zaguan.unizar.es/record/129346/files/texto_completo.pdf Postprint 561100 http://zaguan.unizar.es/record/129346/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:129346 articulos driver 2024-03-18-15:04:30 ARTICLE