000129411 001__ 129411
000129411 005__ 20240319081032.0
000129411 0247_ $$2doi$$a10.1002/anie.202216142
000129411 0248_ $$2sideral$$a135428
000129411 037__ $$aART-2022-135428
000129411 041__ $$aeng
000129411 100__ $$aCheng, Zhiming
000129411 245__ $$aFluorogenic Granzyme A Substrates Enable Real-Time Imaging of Adaptive Immune Cell Activity
000129411 260__ $$c2022
000129411 5060_ $$aAccess copy available to the general public$$fUnrestricted
000129411 5203_ $$aCytotoxic immune cells, including T lymphocytes (CTLs) and natural killer (NK) cells, are essential components of the host response against tumors. CTLs and NK cells secrete granzyme A (GzmA) upon recognition of cancer cells; however, there are very few tools that can detect physiological levels of active GzmA with high spatiotemporal resolution. Herein, we report the rational design of the near‐infrared fluorogenic substrates for human GzmA and mouse GzmA. These activity‐based probes display very high catalytic efficiency and selectivity over other granzymes, as shown in tissue lysates from wild‐type and GzmA knock‐out mice. Furthermore, we demonstrate that the probes can image how adaptive immune cells respond to antigen‐driven recognition of cancer cells in real time.
000129411 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00$$9info:eu-repo/grantAgreement/ES/DGA/B29-20R$$9info:eu-repo/grantAgreement/EC/H2020/859908/EU/Novel Applications in 19F Magnetic Resonance Imaging/NOVA-MRI$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 859908-NOVA-MRI$$9info:eu-repo/grantAgreement/ES/MCIU-AEI/SAF2017-83120-C2-1-R
000129411 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000129411 590__ $$a16.6$$b2022
000129411 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b13 / 178 = 0.073$$c2022$$dQ1$$eT1
000129411 592__ $$a5.573$$b2022
000129411 593__ $$aChemistry (miscellaneous)$$c2022$$dQ1
000129411 593__ $$aCatalysis$$c2022$$dQ1
000129411 594__ $$a26.3$$b2022
000129411 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000129411 700__ $$aThompson, Emily J
000129411 700__ $$aMendive-Tapia, Lorena
000129411 700__ $$aScott, Jamie I
000129411 700__ $$aBenson, Sam
000129411 700__ $$aKitamura, Takanori
000129411 700__ $$aSenan-Salinas, Ana
000129411 700__ $$aSamarakoon, Youhani
000129411 700__ $$aRoberts, Edward W
000129411 700__ $$aArias, Maykel A
000129411 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, Julian$$uUniversidad de Zaragoza
000129411 700__ $$aGalvez, Eva M
000129411 700__ $$aVendrell, Marc
000129411 7102_ $$11011$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Inmunología
000129411 773__ $$g62, 8 (2022), e202216142 [7 pp.]$$pAngew. Chem. (Int. ed.)$$tANGEWANDTE CHEMIE-INTERNATIONAL EDITION$$x1433-7851
000129411 8564_ $$s880813$$uhttps://zaguan.unizar.es/record/129411/files/texto_completo.pdf$$yVersión publicada
000129411 8564_ $$s2939247$$uhttps://zaguan.unizar.es/record/129411/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000129411 909CO $$ooai:zaguan.unizar.es:129411$$particulos$$pdriver
000129411 951__ $$a2024-03-18-17:11:03
000129411 980__ $$aARTICLE