129411 20240319081032.0 doi 10.1002/anie.202216142 sideral 135428 ART-2022-135428 eng Cheng, Zhiming Fluorogenic Granzyme A Substrates Enable Real-Time Imaging of Adaptive Immune Cell Activity 2022 Access copy available to the general public Unrestricted Cytotoxic immune cells, including T lymphocytes (CTLs) and natural killer (NK) cells, are essential components of the host response against tumors. CTLs and NK cells secrete granzyme A (GzmA) upon recognition of cancer cells; however, there are very few tools that can detect physiological levels of active GzmA with high spatiotemporal resolution. Herein, we report the rational design of the near‐infrared fluorogenic substrates for human GzmA and mouse GzmA. These activity‐based probes display very high catalytic efficiency and selectivity over other granzymes, as shown in tissue lysates from wild‐type and GzmA knock‐out mice. Furthermore, we demonstrate that the probes can image how adaptive immune cells respond to antigen‐driven recognition of cancer cells in real time. info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00 info:eu-repo/grantAgreement/ES/DGA/B29-20R info:eu-repo/grantAgreement/EC/H2020/859908/EU/Novel Applications in 19F Magnetic Resonance Imaging/NOVA-MRI This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 859908-NOVA-MRI info:eu-repo/grantAgreement/ES/MCIU-AEI/SAF2017-83120-C2-1-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 16.6 2022 CHEMISTRY, MULTIDISCIPLINARY 13 / 178 = 0.073 2022 Q1 T1 5.573 2022 Chemistry (miscellaneous) 2022 Q1 Catalysis 2022 Q1 26.3 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Thompson, Emily J Mendive-Tapia, Lorena Scott, Jamie I Benson, Sam Kitamura, Takanori Senan-Salinas, Ana Samarakoon, Youhani Roberts, Edward W Arias, Maykel A Pardo, Julian Universidad de Zaragoza (orcid)0000-0003-0154-0730 Galvez, Eva M Vendrell, Marc 1011 566 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Inmunología 62, 8 (2022), e202216142 [7 pp.] Angew. Chem. (Int. ed.) ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 1433-7851 880813 http://zaguan.unizar.es/record/129411/files/texto_completo.pdf Versión publicada 2939247 http://zaguan.unizar.es/record/129411/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:129411 articulos driver 2024-03-18-17:11:03 ARTICLE