000129474 001__ 129474
000129474 005__ 20240319081011.0
000129474 0247_ $$2doi$$a10.1021/acs.bioconjchem.2c00230
000129474 0248_ $$2sideral$$a130339
000129474 037__ $$aART-2022-130339
000129474 041__ $$aeng
000129474 100__ $$0(orcid)0000-0002-3373-9341$$aIdiago-Lopez, Javier
000129474 245__ $$aFrom Bench to Cell: A Roadmap for Assessing the Bioorthogonal "Click" Reactivity of Magnetic Nanoparticles for Cell Surface Engineering
000129474 260__ $$c2022
000129474 5060_ $$aAccess copy available to the general public$$fUnrestricted
000129474 5203_ $$aIn this work, we report the use of bioorthogonal chemistry, specifically the strain-promoted click azide-alkyne cyclo-addition (SPAAC) for the covalent attachment of magnetic nano-particles (MNPs) on living cell membranes. Four types of MNPs were prepared, functionalized with two different stabilizing/passivation agents (a polyethylene glycol derivative and a glucopyranoside derivative, respectively) and two types of strained alkynes with different reactivities: a cyclooctyne (CO) derivative and a dibenzocyclooctyne (DBCO) derivative. The MNPs were extensively characterized in terms of physicochemical characteristics, colloidal stability, and click reactivity in suspension. Then, the reactivity of the MNPs toward azide-modified surfaces was evaluated as a closer approach to their final application in a living cell scenario. Finally, the DBCO-modified MNPs, showing superior reactivity in suspension and on surfaces, were selected for cell membrane immobilization via the SPAAC reaction on the membranes of cells engineered to express azide artificial reporters. Overall, our work provides useful insights into the appropriate surface engineering of nanoparticles to ensure a high performance in terms of bioorthogonal reactivity for biological applications.
000129474 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E15-20R$$9info:eu-repo/grantAgreement/EUR/M-ERA.NET/COFUND-MagicCellGene-PCIN-2017-060$$9info:eu-repo/grantAgreement/ES/MICINN/PGC2018-096016-B-I00$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2017-84246-C2-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/RYC-2015-17640
000129474 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000129474 590__ $$a4.7$$b2022
000129474 591__ $$aCHEMISTRY, ORGANIC$$b8 / 52 = 0.154$$c2022$$dQ1$$eT1
000129474 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b13 / 77 = 0.169$$c2022$$dQ1$$eT1
000129474 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b61 / 178 = 0.343$$c2022$$dQ2$$eT2
000129474 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b92 / 285 = 0.323$$c2022$$dQ2$$eT1
000129474 592__ $$a1.204$$b2022
000129474 593__ $$aBioengineering$$c2022$$dQ1
000129474 593__ $$aBiomedical Engineering$$c2022$$dQ1
000129474 593__ $$aPharmacology$$c2022$$dQ1
000129474 593__ $$aOrganic Chemistry$$c2022$$dQ1
000129474 593__ $$aPharmaceutical Science$$c2022$$dQ1
000129474 593__ $$aBiotechnology$$c2022$$dQ1
000129474 594__ $$a10.1$$b2022
000129474 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000129474 700__ $$0(orcid)0000-0002-8223-3267$$aMoreno-Antolin, Eduardo
000129474 700__ $$aEceiza, Maite
000129474 700__ $$aAizpurua, Jesus M.
000129474 700__ $$0(orcid)0000-0001-6170-4237$$aGrazu, Valeria
000129474 700__ $$0(orcid)0000-0003-1081-8482$$aMartínez de la Fuente, Jesús
000129474 700__ $$0(orcid)0000-0001-5559-8757$$aFratila, Raluca M.
000129474 773__ $$g33 (2022), 1620-1633$$pBioconjug. chem.$$tBioconjugate Chemistry$$x1043-1802
000129474 8564_ $$s2265994$$uhttps://zaguan.unizar.es/record/129474/files/texto_completo.pdf$$yPostprint
000129474 8564_ $$s1046153$$uhttps://zaguan.unizar.es/record/129474/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000129474 909CO $$ooai:zaguan.unizar.es:129474$$particulos$$pdriver
000129474 951__ $$a2024-03-18-15:07:30
000129474 980__ $$aARTICLE