129474 20240319081011.0 doi 10.1021/acs.bioconjchem.2c00230 sideral 130339 ART-2022-130339 eng Idiago-Lopez, Javier (orcid)0000-0002-3373-9341 From Bench to Cell: A Roadmap for Assessing the Bioorthogonal "Click" Reactivity of Magnetic Nanoparticles for Cell Surface Engineering 2022 Access copy available to the general public Unrestricted In this work, we report the use of bioorthogonal chemistry, specifically the strain-promoted click azide-alkyne cyclo-addition (SPAAC) for the covalent attachment of magnetic nano-particles (MNPs) on living cell membranes. Four types of MNPs were prepared, functionalized with two different stabilizing/passivation agents (a polyethylene glycol derivative and a glucopyranoside derivative, respectively) and two types of strained alkynes with different reactivities: a cyclooctyne (CO) derivative and a dibenzocyclooctyne (DBCO) derivative. The MNPs were extensively characterized in terms of physicochemical characteristics, colloidal stability, and click reactivity in suspension. Then, the reactivity of the MNPs toward azide-modified surfaces was evaluated as a closer approach to their final application in a living cell scenario. Finally, the DBCO-modified MNPs, showing superior reactivity in suspension and on surfaces, were selected for cell membrane immobilization via the SPAAC reaction on the membranes of cells engineered to express azide artificial reporters. Overall, our work provides useful insights into the appropriate surface engineering of nanoparticles to ensure a high performance in terms of bioorthogonal reactivity for biological applications. info:eu-repo/grantAgreement/ES/DGA/E15-20R info:eu-repo/grantAgreement/EUR/M-ERA.NET/COFUND-MagicCellGene-PCIN-2017-060 info:eu-repo/grantAgreement/ES/MICINN/PGC2018-096016-B-I00 info:eu-repo/grantAgreement/ES/MINECO/BIO2017-84246-C2-1-R info:eu-repo/grantAgreement/ES/MINECO/RYC-2015-17640 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.7 2022 CHEMISTRY, ORGANIC 8 / 52 = 0.154 2022 Q1 T1 BIOCHEMICAL RESEARCH METHODS 13 / 77 = 0.169 2022 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 61 / 178 = 0.343 2022 Q2 T2 BIOCHEMISTRY & MOLECULAR BIOLOGY 92 / 285 = 0.323 2022 Q2 T1 1.204 2022 Bioengineering 2022 Q1 Biomedical Engineering 2022 Q1 Pharmacology 2022 Q1 Organic Chemistry 2022 Q1 Pharmaceutical Science 2022 Q1 Biotechnology 2022 Q1 10.1 2022 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Moreno-Antolin, Eduardo (orcid)0000-0002-8223-3267 Eceiza, Maite Aizpurua, Jesus M. Grazu, Valeria (orcid)0000-0001-6170-4237 Martínez de la Fuente, Jesús (orcid)0000-0003-1081-8482 Fratila, Raluca M. (orcid)0000-0001-5559-8757 33 (2022), 1620-1633 Bioconjug. chem. Bioconjugate Chemistry 1043-1802 2265994 http://zaguan.unizar.es/record/129474/files/texto_completo.pdf Postprint 1046153 http://zaguan.unizar.es/record/129474/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:129474 articulos driver 2024-03-18-15:07:30 ARTICLE