000129504 001__ 129504
000129504 005__ 20231219145829.0
000129504 0247_ $$2doi$$a10.1016/j.aca.2013.10.016
000129504 0248_ $$2sideral$$a83921
000129504 037__ $$aART-2013-83921
000129504 041__ $$aeng
000129504 100__ $$aGarcia Otero, N.
000129504 245__ $$aAssessment of metals bound to marine plankton proteins and to dissolved proteins in seawater
000129504 260__ $$c2013
000129504 5060_ $$aAccess copy available to the general public$$fUnrestricted
000129504 5203_ $$aStudies based on laser ablation–inductively coupled plasma-mass spectrometry (LA–ICP-MS) have been performed to assess metal bound to dissolved proteins and proteins from marine plankton after two-dimensional polyacrylamide gel electrophoresis (2D PAGE). Dissolved proteins were pre-concentrated from surface seawater (60 L) by tangential ultrafiltration with 10 kDa molecular weight cut-off (MWCO) membranes and further centrifugal ultrafiltration (10 kDa) before proteins isolation by methanol/chloroform/water precipitation. Proteins isolation from plankton was assessed after different trichloroacetic acid (TCA)/acetone and methanol washing stages, and further proteins extraction with a phenol solution. LA–ICP-MS analysis of the electrophoretic profiles obtained for dissolved proteins shows the presence of Cd, Cr, Cu, and Zn in five spots analyzed. These proteins exhibit quite similar molecular weights (within the 10–14 kDa range) and pIs (from 5.8 to 7.3). Cd, Cr, Cu, and Zn have also been found to be associated to proteins isolated from plankton samples. In this case, Cd has been found to be bound to proteins of quite different molecular weight (9, 13 and 22 kDa) and pIs (4.5, 5.2, 5.5, and 10). However, trace elements such as Cr, Cu and Zn appear to be mainly bound to plankton proteins of low molecular weight and variable pI.
000129504 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-38091-C02-02
000129504 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000129504 590__ $$a4.517$$b2013
000129504 591__ $$aCHEMISTRY, ANALYTICAL$$b5 / 75 = 0.067$$c2013$$dQ1$$eT1
000129504 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000129504 700__ $$aBarciela Alonso, M.C.
000129504 700__ $$aMoreda Piñeiro, A.
000129504 700__ $$aBermejo Barrera, P.
000129504 700__ $$0(orcid)0000-0002-8460-6020$$aJiménez, M.S.$$uUniversidad de Zaragoza
000129504 700__ $$0(orcid)0000-0003-1400-9041$$aGómez Cotín, M.T.$$uUniversidad de Zaragoza
000129504 700__ $$0(orcid)0000-0002-4070-8607$$aCastillo, J.R.$$uUniversidad de Zaragoza
000129504 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000129504 773__ $$g804 (2013), 59-65$$pAnal. chim. acta$$tAnalytica Chimica Acta$$x0003-2670
000129504 8564_ $$s203507$$uhttps://zaguan.unizar.es/record/129504/files/texto_completo.pdf$$yPostprint
000129504 8564_ $$s1407793$$uhttps://zaguan.unizar.es/record/129504/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000129504 909CO $$ooai:zaguan.unizar.es:129504$$particulos$$pdriver
000129504 951__ $$a2023-12-19-14:02:30
000129504 980__ $$aARTICLE