129504 20231219145829.0 doi 10.1016/j.aca.2013.10.016 sideral 83921 ART-2013-83921 eng Garcia Otero, N. Assessment of metals bound to marine plankton proteins and to dissolved proteins in seawater 2013 Studies based on laser ablation–inductively coupled plasma-mass spectrometry (LA–ICP-MS) have been performed to assess metal bound to dissolved proteins and proteins from marine plankton after two-dimensional polyacrylamide gel electrophoresis (2D PAGE). Dissolved proteins were pre-concentrated from surface seawater (60 L) by tangential ultrafiltration with 10 kDa molecular weight cut-off (MWCO) membranes and further centrifugal ultrafiltration (10 kDa) before proteins isolation by methanol/chloroform/water precipitation. Proteins isolation from plankton was assessed after different trichloroacetic acid (TCA)/acetone and methanol washing stages, and further proteins extraction with a phenol solution. LA–ICP-MS analysis of the electrophoretic profiles obtained for dissolved proteins shows the presence of Cd, Cr, Cu, and Zn in five spots analyzed. These proteins exhibit quite similar molecular weights (within the 10–14 kDa range) and pIs (from 5.8 to 7.3). Cd, Cr, Cu, and Zn have also been found to be associated to proteins isolated from plankton samples. In this case, Cd has been found to be bound to proteins of quite different molecular weight (9, 13 and 22 kDa) and pIs (4.5, 5.2, 5.5, and 10). However, trace elements such as Cr, Cu and Zn appear to be mainly bound to plankton proteins of low molecular weight and variable pI. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-38091-C02-02 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 4.517 2013 CHEMISTRY, ANALYTICAL 5 / 75 = 0.067 2013 Q1 T1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Barciela Alonso, M.C. Moreda Piñeiro, A. Bermejo Barrera, P. Jiménez, M.S. Universidad de Zaragoza (orcid)0000-0002-8460-6020 Gómez Cotín, M.T. Universidad de Zaragoza (orcid)0000-0003-1400-9041 Castillo, J.R. Universidad de Zaragoza (orcid)0000-0002-4070-8607 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 804 (2013), 59-65 Anal. chim. acta Analytica Chimica Acta 0003-2670 203507 http://zaguan.unizar.es/record/129504/files/texto_completo.pdf Postprint 1407793 http://zaguan.unizar.es/record/129504/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:129504 articulos driver 2023-12-19-14:02:30 ARTICLE