A Flexible PDMS-Based Optical Biosensor for Stretch Monitoring in Cardiac Tissue Samples

Sannino, Andrea (Universidad de Zaragoza) ; Velarte, Antonio (Universidad de Zaragoza) ; Otín, Aránzazu (Universidad de Zaragoza) ; Artigas, José Ignacio (Universidad de Zaragoza) ; Oliván-Viguera, Aida

Resumen: Cardiotoxicity, characterized by adverse impacts on normal heart function due to drug exposure, is a significant concern due to the potentially serious side effects associated with various pharmaceuticals. It is essential to detect the cardiotoxicity of a drug as early as possible in the testing phase of a medical composite. Therefore, there is a pressing need for more reliable in vitro models that accurately mimic the in vivo conditions of cardiac biopsies. In a functional beating heart, cardiac muscle cells are under the effect of static and cyclic stretches. It has been demonstrated that cultured cardiac biopsies can benefit from external mechanical loads that resemble the in vivo condition, increasing the probability of cardiotoxicity detection in the early testing stages. In this work, a biosensor is designed and fabricated to allow for stretch monitoring in biopsies and tissue cultures using an innovative sensing mechanism. The detection setup is based on a biocompatible, thin, flexible membrane—where the samples are attached—which is used as an optical waveguide to detect pressure-caused shape changes and stretches. Various prototypes have been fabricated with a cost-effective process, and different measurements have been carried out to experimentally validate the proposed measurement technique. From these evaluations, stretches of up to 1.5% have been measured, but the performed simulations point towards the possibility of expanding the considered technique up to 10–30% stretches.

Idioma: Inglés

DOI: 10.3390/s23239454

Año: 2023

Publicado en: Sensors 23, 23 (2023), 9454 [17 pp.]
ISSN: 1424-8220

Financiación: info:eu-repo/grantAgreement/ES/DGA/GEPM T23-23
Financiación: info:eu-repo/grantAgreement/ES/DGA/LMP94_21
Financiación: info:eu-repo/grantAgreement/ES/DGA/T39-23R
Financiación: info:eu-repo/grantAgreement/EC/H2020/638284/EU/Is your heart aging well? A systems biology approach to characterize cardiac aging from the cell to the body surface/MODELAGE
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2019-105674RB-I00
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2022-140556OB-I00
Financiación: info:eu-repo/grantAgreement/EUR/MICINN/TED2021-130459B-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Tecnología Electrónica (Dpto. Ingeniería Electrón.Com.)
Área (Departamento): Área Teoría Señal y Comunicac. (Dpto. Ingeniería Electrón.Com.)