129609 20240104102231.0 doi 10.1002/cbin.10865 sideral 103522 ART-2017-103522 eng Gasco, S. Comparative study of hematopoietic stem and progenitor cells between sexes in mice under physiological conditions along time 2017 Hematopoietic stem and progenitor cells (HSPCs) are attractive targets in regenerative medicine, although the differences in their homeostatic maintenance between sexes along time are still under debate. We accurately monitored hematopoietic stem cells (HSCs), common lymphoid progenitors (CLPs), and common myeloid progenitors (CMPs) frequencies by flow cytometry, by performing serial peripheral blood extractions from male and female B6SJL wild-type mice and found no significant differences. Only modest differences were found in the gene expression profile of Slamf1 and Gata2. Our findings suggest that both sexes could be used indistinctly to perform descriptive studies in the murine hematopoietic system, especially for flow cytometry studies in peripheral blood. This would allow diminishing the number of animals needed for the experimental procedures. In addition, the use of serial extractions in the same animals drastically decreases the number of animals needed. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/FIS/PI14-00947 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 1.936 2017 CELL BIOLOGY 153 / 190 = 0.805 2017 Q4 T3 0.712 2017 Medicine (miscellaneous) 2017 Q2 Cell Biology 2017 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Rando, A. (orcid)0000-0002-2898-4561 Zaragoza, P. Universidad de Zaragoza (orcid)0000-0001-5740-0185 García-Redondo, A. Calvo, A.C. Universidad de Zaragoza (orcid)0000-0001-5193-7782 Osta, R. Universidad de Zaragoza (orcid)0000-0001-5687-6704 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 41, 12 (2017), 1399-1405 Cell biol. int. CELL BIOLOGY INTERNATIONAL 1065-6995 251882 http://zaguan.unizar.es/record/129609/files/texto_completo.pdf Postprint 2386215 http://zaguan.unizar.es/record/129609/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:129609 articulos driver 2024-01-04-09:04:52 ARTICLE