000129610 001__ 129610
000129610 005__ 20240104102231.0
000129610 0247_ $$2doi$$a10.1042/BCJ20170692
000129610 0248_ $$2sideral$$a103894
000129610 037__ $$aART-2018-103894
000129610 041__ $$aeng
000129610 100__ $$aSein-Echaluce, Violeta C.
000129610 245__ $$aMolecular basis for the integration of environmental signals by FurB from Anabaena sp. PCC 7120
000129610 260__ $$c2018
000129610 5060_ $$aAccess copy available to the general public$$fUnrestricted
000129610 5203_ $$aFUR (Ferric uptake regulator) proteins are among the most important families of transcriptional regulators in prokaryotes, often behaving as global regulators. In the cyanobacterium Anabaena PCC 7120, FurB (Zur, Zinc uptake regulator) controls zinc and redox homeostasis through the repression of target genes in a zinc-dependent manner. In vitro, non-specific binding of FurB to DNA elicits protection against oxidative damage and avoids cleavage by deoxyribonuclease I. The present study provides, for the first time, evidence of the influence of redox environment in the interaction of FurB with regulatory zinc and its consequences in FurB–DNA-binding affinity. Calorimetry studies showed that, in addition to one structural Zn(II), FurB is able to bind two additional Zn(II) per monomer and demonstrated the implication of cysteine C93 in regulatory Zn(II) coordination. The interaction of FurB with the second regulatory zinc occurred only under reducing conditions. While non-specific FurB–DNA interaction is Zn(II)-independent, the optimal binding of FurB to target promoters required loading of two regulatory zinc ions. Those results combined with site-directed mutagenesis and gel-shift assays evidenced that the redox state of cysteine C93 conditions the binding of the second regulatory Zn(II) and, in turn, modulates the affinity for a specific DNA target. Furthermore, differential spectroscopy studies showed that cysteine C93 could also be involved in heme coordination by FurB, either as a direct ligand or being located near the binding site. The results indicate that besides controlling zinc homeostasis, FurB could work as a redox-sensing protein probably modifying its zinc and DNA-binding abilities depending upon environmental conditions.
000129610 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B18$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2012-31458$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2016-77671-P$$9info:eu-repo/grantAgreement/ES/UZ/UZ-2016-BIO-02
000129610 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000129610 590__ $$a4.331$$b2018
000129610 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b72 / 294 = 0.245$$c2018$$dQ1$$eT1
000129610 592__ $$a2.142$$b2018
000129610 593__ $$aBiochemistry$$c2018$$dQ1
000129610 593__ $$aMolecular Biology$$c2018$$dQ1
000129610 593__ $$aCell Biology$$c2018$$dQ1
000129610 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000129610 700__ $$aPallarés, María Carmen
000129610 700__ $$0(orcid)0000-0001-7460-5916$$aLostao, Anabel$$uUniversidad de Zaragoza
000129610 700__ $$0(orcid)0000-0003-3608-4720$$aYruela, Inmaculada$$uUniversidad de Zaragoza
000129610 700__ $$0(orcid)0000-0001-5702-4538$$aVelázquez-Campoy, Adrián$$uUniversidad de Zaragoza
000129610 700__ $$0(orcid)0000-0002-2742-3711$$aPeleato, M. Luisa$$uUniversidad de Zaragoza
000129610 700__ $$0(orcid)0000-0001-8644-4574$$aFillat, Maria F.$$uUniversidad de Zaragoza
000129610 7102_ $$11002$$2412$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Fisiología Vegetal
000129610 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000129610 773__ $$g475, 1 (2018), 151-168$$pBiochem. j.$$tBiochemical Journal$$x0264-6021
000129610 8564_ $$s2240753$$uhttps://zaguan.unizar.es/record/129610/files/texto_completo.pdf$$yPostprint
000129610 8564_ $$s1178012$$uhttps://zaguan.unizar.es/record/129610/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000129610 909CO $$ooai:zaguan.unizar.es:129610$$particulos$$pdriver
000129610 951__ $$a2024-01-04-09:04:55
000129610 980__ $$aARTICLE