The correlation of two different real-time PCR devices for the analysis of CYP2C19 pharmacogenetic results

Alonso Llorente, Alba ; Salgado Garrido, Josefa ; Teijido Hermida, Oscar ; González Andrade, Fabricio ; Valiente Martín, Alberto ; Fanlo Villacampa, Ana (Universidad de Zaragoza) ; Vicente Romero, Jorge (Universidad de Zaragoza)

Resumen: CYP2C19 is a highly polymorphic gene responsible for the metabolism of commonly used drugs. CYP2C19*1, the wild-type allele, is associated with normal enzyme activity, whereas CYP2C19*2 and CYP2C19*17 lead to null and increased enzyme activity, respectively. The use of different instruments to perform the same pharmacogenetic tests should not affect the reliability of the results reported to clinicians, as required by the ISO 15189 standard. Genotyping assays allowed for the identification of gene variants corresponding to the CYP2C19*2 and CYP2C19*17 haplotypes in 44 selected samples. Each sample was analyzed in duplicate using the Thermo Fisher Taqman Drug Metabolism probes CYP2C19*2: c_25986767_70 (rs4244285) and CYP2C19*17: c_469857_10 (rs12248560). The experiments were performed on two widely used types of real-time PCR analyzers: ABI PRSIM™7500 and QuantStudioTM12KFlex (both from Applied Biosystems, Thermofisher). The data were analyzed in a Thermo Fisher Cloud facility. The analysis was performed independently by two qualified professionals. Both different instruments and analysts’ interpretations were consistent in identifying the native homozygous, heterozygous, and mutant homozygous variants for CYP2C19*2 and CYP2C19*17. The results provided by both the primary and backup analyzers showed a perfect correlation. This would allow for the use of the backup analyzer in case the main one is not available.

Idioma: Inglés

DOI: 10.3390/scipharm91040051

Año: 2023

Publicado en: Scientia Pharmaceutica 91, 4 (2023), 51 [7 pp.]
ISSN: 0036-8709

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Farmacología (Dpto. Farmac.Fisiol.y Med.L.F.)