129659 20240731103327.0 doi 10.3390/scipharm91040051 sideral 135873 ART-2023-135873 eng Alonso Llorente, Alba The correlation of two different real-time PCR devices for the analysis of CYP2C19 pharmacogenetic results 2023 Access copy available to the general public Unrestricted CYP2C19 is a highly polymorphic gene responsible for the metabolism of commonly used drugs. CYP2C19*1, the wild-type allele, is associated with normal enzyme activity, whereas CYP2C19*2 and CYP2C19*17 lead to null and increased enzyme activity, respectively. The use of different instruments to perform the same pharmacogenetic tests should not affect the reliability of the results reported to clinicians, as required by the ISO 15189 standard. Genotyping assays allowed for the identification of gene variants corresponding to the CYP2C19*2 and CYP2C19*17 haplotypes in 44 selected samples. Each sample was analyzed in duplicate using the Thermo Fisher Taqman Drug Metabolism probes CYP2C19*2: c_25986767_70 (rs4244285) and CYP2C19*17: c_469857_10 (rs12248560). The experiments were performed on two widely used types of real-time PCR analyzers: ABI PRSIM™7500 and QuantStudioTM12KFlex (both from Applied Biosystems, Thermofisher). The data were analyzed in a Thermo Fisher Cloud facility. The analysis was performed independently by two qualified professionals. Both different instruments and analysts’ interpretations were consistent in identifying the native homozygous, heterozygous, and mutant homozygous variants for CYP2C19*2 and CYP2C19*17. The results provided by both the primary and backup analyzers showed a perfect correlation. This would allow for the use of the backup analyzer in case the main one is not available. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 0.412 2023 Pharmaceutical Science 2023 Q2 4.6 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Salgado Garrido, Josefa Teijido Hermida, Oscar González Andrade, Fabricio Valiente Martín, Alberto Fanlo Villacampa, Ana Universidad de Zaragoza (orcid)0000-0001-8064-8138 Vicente Romero, Jorge Universidad de Zaragoza (orcid)0000-0003-4629-6743 1012 315 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Farmacología 91, 4 (2023), 51 [7 pp.] Scientia Pharmaceutica 0036-8709 502227 http://zaguan.unizar.es/record/129659/files/texto_completo.pdf Versión publicada 2759294 http://zaguan.unizar.es/record/129659/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:129659 articulos driver 2024-07-31-09:45:10 ARTICLE