| Página principal > Artículos > Validation of an EIA [enzyme immunoassay] technique for the determination of salivary cortisol in cattle > MARC |
000129684 001__ 129684 000129684 005__ 20240104102233.0 000129684 0247_ $$2doi$$a10.5424/sjar/2004021-59 000129684 0248_ $$2sideral$$a55120 000129684 037__ $$aART-2004-55120 000129684 041__ $$aeng 000129684 100__ $$aChacón Pérez, G. 000129684 245__ $$aValidation of an EIA [enzyme immunoassay] technique for the determination of salivary cortisol in cattle 000129684 260__ $$c2004 000129684 5060_ $$aAccess copy available to the general public$$fUnrestricted 000129684 5203_ $$aThis work involves the development and validation of an enzyme immunoassay technique (EIA) for the measurement of the cortisol concentration in cattle saliva. Saliva samples present several advantages over plasma samples in animal welfare studies. Saliva collection avoids venipuncture as a stress factor. Also, saliva components do not affect EIA as plasma components do. At present, there is no validated commercial method for saliva cortisol determination in cattle. Commercially available radioimmunoassay kits for human plasma (detection range: 10-100 ng ml -1 ) are not sensitive enough for animals with low concentrations of salivary cortisol (< 4 ng ml -1 ). Thus, EIA is the method of choice in cattle. Sensitivity, specificity, precision and accuracy EIA tests showed this method to be suitable and reliable. The detection limit was found to be 0.024 ng ml -1 , representing an improvement on previously described techniques. Intra-assay and inter-assay variation coefficients were 1.47-7.30% and 2.40-9.78%, respectively. The recovery rates for cortisol added to saliva samples were 91.36-126.5%. Parallelism tests showed that saliva cortisol levels can be determined in cattle samples without extraction. The correlation between saliva and plasma cortisol was positive (r = 0.75) and the saliva/plasma cortisol ratio was around 10%. Therefore, saliva samples are a suitable alternative to plasma samples in bovine HPA (hypothalamic-pituitary-adrenal) axis evaluation 000129684 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000129684 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000129684 700__ $$0(orcid)0000-0001-5084-6555$$aGarcía Belenguer Laita, S.$$uUniversidad de Zaragoza 000129684 700__ $$aIllera del Portal, J.C. 000129684 700__ $$0(orcid)0000-0002-1581-3085$$aPalacio Liesa, J.$$uUniversidad de Zaragoza 000129684 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000129684 773__ $$g2, 1 (2004), 45-51$$pSpan. j. agric. res.$$tSpanish Journal of Agricultural Research$$x1695-971X 000129684 8564_ $$s49769$$uhttps://zaguan.unizar.es/record/129684/files/texto_completo.pdf$$yVersión publicada 000129684 8564_ $$s2608090$$uhttps://zaguan.unizar.es/record/129684/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000129684 909CO $$ooai:zaguan.unizar.es:129684$$particulos$$pdriver 000129684 951__ $$a2024-01-04-09:09:10 000129684 980__ $$aARTICLE
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