000129768 001__ 129768
000129768 005__ 20240705134208.0
000129768 0247_ $$2doi$$a10.1007/s00253-023-12840-6
000129768 0248_ $$2sideral$$a136085
000129768 037__ $$aART-2024-136085
000129768 041__ $$aeng
000129768 100__ $$0(orcid)0000-0003-3752-4660$$aPagan, Elisa
000129768 245__ $$aAdaptive evolution of Salmonella Typhimurium LT2 exposed to carvacrol lacks a uniform pattern
000129768 260__ $$c2024
000129768 5060_ $$aAccess copy available to the general public$$fUnrestricted
000129768 5203_ $$aEmergence of genetic variants with increased resistance/tolerance to natural antimicrobials, such as essential oils, has been previously evidenced; however, it is unknown whether mutagenesis follows a general or a specific pattern. For this purpose, we carried out four adaptive laboratory evolutions (ALE) in parallel of Salmonella enterica Typhimurium with carvacrol. After 10 evolution steps, we selected and characterized one colony from each lineage (SeCarA, SeCarB, SeCarC, and SeCarD). Phenotypic characterization of the four evolved strains revealed enhanced survival to lethal treatments; two of them (SeCarA and SeCarB) showed an increase of minimum inhibitory concentration of carvacrol and a better growth fitness in the presence of carvacrol compared to wild-type strain. Whole genome sequencing revealed 10 mutations, of which four (rrsH, sseG, wbaV, and flhA) were present in more than one strain, whereas six (nirC, fliH, lon, rob, upstream yfhP, and upstream argR) were unique to individual strains. Single-mutation genetic constructs in SeWT confirmed lon and rob as responsible for the increased resistance to carvacrol as well as to antibiotics (ampicillin, ciprofloxacin, chloramphenicol, nalidixic acid, rifampicin, tetracycline, and trimethoprim). wbaV played an important role in increased tolerance against carvacrol and chloramphenicol, and flhA in cross-tolerance to heat treatments. As a conclusion, no common phenotypical or genotypical pattern was observed in the isolated resistant variants of Salmonella Typhimurium emerged under carvacrol stress. Furthermore, the demonstration of cross-resistance against heat and antibiotics exhibited by resistant variants raises concerns regarding food safety.
000129768 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/PGC2018-093789-B-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-123404NB-I00$$9info:eu-repo/grantAgreement/ES/MEC/FPU17-02441
000129768 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000129768 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000129768 700__ $$0(orcid)0000-0003-4460-3543$$aMerino, Natalia$$uUniversidad de Zaragoza
000129768 700__ $$0(orcid)0000-0001-5053-8309$$aBerdejo, Daniel$$uUniversidad de Zaragoza
000129768 700__ $$0(orcid)0000-0003-2579-5375$$aCampillo, Raul$$uUniversidad de Zaragoza
000129768 700__ $$0(orcid)0000-0002-5895-2157$$aGayan, Elisa$$uUniversidad de Zaragoza
000129768 700__ $$0(orcid)0000-0002-7629-8101$$aGarcía-Gonzalo, Diego$$uUniversidad de Zaragoza
000129768 700__ $$0(orcid)0000-0002-0238-6328$$aPagan, Rafael$$uUniversidad de Zaragoza
000129768 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología
000129768 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000129768 773__ $$g108, 1 (2024), [14 pp.]$$pAppl. microbiol. biotechnol.$$tAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY$$x0175-7598
000129768 8564_ $$s1228771$$uhttps://zaguan.unizar.es/record/129768/files/texto_completo.pdf$$yVersión publicada
000129768 8564_ $$s2347656$$uhttps://zaguan.unizar.es/record/129768/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000129768 909CO $$ooai:zaguan.unizar.es:129768$$particulos$$pdriver
000129768 951__ $$a2024-07-05-12:51:02
000129768 980__ $$aARTICLE