000129826 001__ 129826
000129826 005__ 20241125101147.0
000129826 0247_ $$2doi$$a10.1021/acs.bioconjchem.3c00417
000129826 0248_ $$2sideral$$a136133
000129826 037__ $$aART-2023-136133
000129826 041__ $$aeng
000129826 100__ $$aCastro-Hinojosa, Christian
000129826 245__ $$aA simple and versatile strategy for oriented immobilization of his-tagged proteins on magnetic nanoparticles
000129826 260__ $$c2023
000129826 5060_ $$aAccess copy available to the general public$$fUnrestricted
000129826 5203_ $$aOriented and covalent immobilization of proteins on magnetic nanoparticles (MNPs) is particularly challenging as it requires both the functionality of the protein and the colloidal stability of the MNPs to be preserved. Here, we describe a simple, straightforward, and efficient strategy for MNP functionalization with proteins using metal affinity binding. Our method involves a single-step process where MNPs are functionalized using a preformed, ready-to-use nitrilotriacetic acid-divalent metal cation (NTA-M2+) complex and polyethylene glycol (PEG) molecules. As a proof-of-concept, we demonstrate the oriented immobilization of a recombinant cadherin fragment engineered with a hexahistidine tag (6His-tag) onto the MNPs. Our developed methodology is simple and direct, enabling the oriented bioconjugation of His-tagged cadherins to MNPs while preserving protein functionality and the colloidal stability of the MNPs, and could be extended to other proteins expressing a polyhistidine tag. When compared to the traditional method where NTA is first conjugated to the MNPs and afterward free metal ions are added to form the complex, this novel strategy results in a higher functionalization efficiency while avoiding MNP aggregation. Additionally, our method allows for covalent bonding of the cadherin fragments to the MNP surface while preserving functionality, making it highly versatile. Finally, our strategy not only ensures the correct orientation of the protein fragments on the MNPs but also allows for the precise control of their density. This feature enables the selective targeting of E-cadherin-expressing cells only when MNPs are decorated with a high density of cadherin fragments.
000129826 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E15-20R$$9info:eu-repo/grantAgreement/EC/H2020/853468/EU/Remote control of cellular signalling triggered by magnetic switching/SIROCCO$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 853468-SIROCCO$$9info:eu-repo/grantAgreement/ES/MECD/FPU17-02024$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PRTR-C17.I1$$9info:eu-repo/grantAgreement/ES/MICINN/PGC2018-096016-B-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-122508NB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2015-17640$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2019-026860-I
000129826 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000129826 590__ $$a4.0$$b2023
000129826 592__ $$a1.085$$b2023
000129826 591__ $$aCHEMISTRY, ORGANIC$$b9 / 58 = 0.155$$c2023$$dQ1$$eT1
000129826 593__ $$aBioengineering$$c2023$$dQ1
000129826 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b14 / 85 = 0.165$$c2023$$dQ1$$eT1
000129826 593__ $$aBiomedical Engineering$$c2023$$dQ1
000129826 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b79 / 231 = 0.342$$c2023$$dQ2$$eT2
000129826 593__ $$aPharmacology$$c2023$$dQ1
000129826 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b100 / 313 = 0.319$$c2023$$dQ2$$eT1
000129826 593__ $$aOrganic Chemistry$$c2023$$dQ1
000129826 593__ $$aPharmaceutical Science$$c2023$$dQ1
000129826 593__ $$aBiotechnology$$c2023$$dQ1
000129826 594__ $$a9.0$$b2023
000129826 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000129826 700__ $$aDel Sol-Fernández, Susel
000129826 700__ $$0(orcid)0000-0002-8223-3267$$aMoreno-Antolín, Eduardo
000129826 700__ $$0(orcid)0000-0002-3770-4867$$aMartín-Gracia, Beatriz
000129826 700__ $$aOvejero, Jesús G.
000129826 700__ $$0(orcid)0000-0003-1081-8482$$aMartínez de la Fuente, Jesús
000129826 700__ $$0(orcid)0000-0001-6170-4237$$aGrazú, Valeria
000129826 700__ $$0(orcid)0000-0001-5559-8757$$aFratila, Raluca M.$$uUniversidad de Zaragoza
000129826 700__ $$0(orcid)0000-0002-2861-2469$$aMoros, María
000129826 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000129826 773__ $$g34, 12 (2023), 2275-2292$$pBioconjug. chem.$$tBioconjugate Chemistry$$x1043-1802
000129826 8564_ $$s9137442$$uhttps://zaguan.unizar.es/record/129826/files/texto_completo.pdf$$yVersión publicada
000129826 8564_ $$s3398819$$uhttps://zaguan.unizar.es/record/129826/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000129826 909CO $$ooai:zaguan.unizar.es:129826$$particulos$$pdriver
000129826 951__ $$a2024-11-22-12:04:58
000129826 980__ $$aARTICLE