000129903 001__ 129903
000129903 005__ 20241125101147.0
000129903 0247_ $$2doi$$a10.1186/s13567-023-01211-8
000129903 0248_ $$2sideral$$a136137
000129903 037__ $$aART-2023-136137
000129903 041__ $$aeng
000129903 100__ $$aSola, Diego$$uUniversidad de Zaragoza
000129903 245__ $$aNovel polymorphisms in the prion protein gene (PRNP) and stability of the resultant prion protein in different horse breeds
000129903 260__ $$c2023
000129903 5060_ $$aAccess copy available to the general public$$fUnrestricted
000129903 5203_ $$aPrion diseases are fatal neurodegenerative disorders in which the main pathogenic event is the conversion of the cellular prion protein (PrPC) into an abnormal and misfolded isoform known as PrPSc. Most prion diseases and their susceptibility and pathogenesis are mainly modulated by the PRNP gene that codes for PrP. Mutations and polymorphisms in the PRNP gene can alter PrPC amino acid sequence, leading to a change in transmission efficiency depending on the place where it occurs. Horses are animals that are considered to be highly resistant to prions. Several studies have attempted to identify polymorphisms in the PRNP gene that explain the reason for this high resistance. In this study, we have analysed 207 horses from 20 different breeds, discovering 3 novel PRNP polymorphisms. By using computer programmes such as PolyPhen-2, PROVEAN, PANTHER, Meta-SNP and PredictSNP, we have predicted the possible impact that these new polymorphisms would have on the horse prion protein. In addition, we measured the propensity for amyloid aggregation using AMYCO and analysed the lack of hydrogen bridges that these changes would entail together with their electrostatic potentials using Swiss-PdbViewer software, showing that an increased amyloid propensity could be due to changes at the level of electrostatic potentials.
000129903 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A05-20R
000129903 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000129903 590__ $$a3.7$$b2023
000129903 592__ $$a0.907$$b2023
000129903 591__ $$aVETERINARY SCIENCES$$b10 / 167 = 0.06$$c2023$$dQ1$$eT1
000129903 593__ $$aVeterinary (miscellaneous)$$c2023$$dQ1
000129903 594__ $$a7.0$$b2023
000129903 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000129903 700__ $$aArtigas, Rody
000129903 700__ $$0(orcid)0000-0002-8692-1382$$aMediano, Diego R.
000129903 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza
000129903 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan José$$uUniversidad de Zaragoza
000129903 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, Inmaculada$$uUniversidad de Zaragoza
000129903 700__ $$0(orcid)0000-0001-5105-6133$$aAcín, Cristina$$uUniversidad de Zaragoza
000129903 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000129903 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000129903 773__ $$g54, 94 (2023), 1-10$$pVet. res.$$tVeterinary Research$$x0928-4249
000129903 8564_ $$s1909873$$uhttps://zaguan.unizar.es/record/129903/files/texto_completo.pdf$$yVersión publicada
000129903 8564_ $$s2520668$$uhttps://zaguan.unizar.es/record/129903/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000129903 909CO $$ooai:zaguan.unizar.es:129903$$particulos$$pdriver
000129903 951__ $$a2024-11-22-12:05:04
000129903 980__ $$aARTICLE