129915 20241125101148.0 doi 10.1186/s13567-023-01225-2 sideral 136249 ART-2023-136249 eng Betancor, Marina Universidad de Zaragoza (orcid)0000-0002-0388-9429 Detection of classical BSE prions in asymptomatic cows after inoculation with atypical/Nor98 scrapie 2023 Access copy available to the general public Unrestricted The emergence of bovine spongiform encephalopathy (BSE) prions from atypical scrapie has been recently observed upon experimental transmission to rodent and swine models. This study aimed to assess whether the inoculation of atypical scrapie could induce BSE-like disease in cattle. Four calves were intracerebrally challenged with atypical scrapie. Animals were euthanized without clinical signs of prion disease and tested negative for PrPSc accumulation by immunohistochemistry and western blotting. However, an emergence of BSE-like prion seeding activity was detected during in vitro propagation of brain samples from the inoculated animals. These findings suggest that atypical scrapie may represent a potential source of BSE infection in cattle. info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA-148-16 REDPRION info:eu-repo/grantAgreement/ES/MICINN/PID2021-125398OB-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.7 2023 VETERINARY SCIENCES 10 / 167 = 0.06 2023 Q1 T1 0.907 2023 Veterinary (miscellaneous) 2023 Q1 7.0 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Marín, Belén Universidad de Zaragoza (orcid)0000-0002-1590-3347 Otero, Alicia Universidad de Zaragoza (orcid)0000-0001-9075-2764 Hedman, Carlos Universidad de Zaragoza (orcid)0000-0002-0827-110X Romero, Antonio Universidad de Zaragoza (orcid)0000-0001-7188-0461 Barrio, Tomás Sevilla, Eloisa Universidad de Zaragoza (orcid)0000-0002-4213-2904 Douet, Jean-Yves Huor, Alvina Badiola, Juan José Universidad de Zaragoza (orcid)0000-0002-7173-7216 Andréoletti, Olivier Bolea, Rosa Universidad de Zaragoza (orcid)0000-0002-2746-3932 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 54, 89 (2023), 1-7 Vet. res. Veterinary Research 0928-4249 972767 http://zaguan.unizar.es/record/129915/files/texto_completo.pdf Versión publicada 2570530 http://zaguan.unizar.es/record/129915/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:129915 articulos driver 2024-11-22-12:05:33 ARTICLE