000129921 001__ 129921
000129921 005__ 20240705134210.0
000129921 0247_ $$2doi$$a10.3390/plants13020192
000129921 0248_ $$2sideral$$a136140
000129921 037__ $$aART-2024-136140
000129921 041__ $$aeng
000129921 100__ $$aFerrando, Natalia
000129921 245__ $$aEnhancing commercial antibiotics with trans-cinnamaldehyde in gram-positive and gram-negative bacteria: an in vitro approach
000129921 260__ $$c2024
000129921 5060_ $$aAccess copy available to the general public$$fUnrestricted
000129921 5203_ $$aOne strategy to mitigate the emergence of bacterial resistance involves reducing antibiotic doses by combining them with natural products, such as trans-cinnamaldehyde (CIN). The objective of this research was to identify in vitro combinations (CIN + commercial antibiotic (ABX)) that decrease the minimum inhibitory concentration (MIC) of seven antibiotics against 14 different Grampositive and Gram-negative pathogenic bacteria, most of them classified as ESKAPE. MIC values were measured for all compounds using the broth microdilution method. The effect of the combinations on these microorganisms was analyzed through the checkboard assay to determine the type of activity (synergy, antagonism, or addition). This analysis was complemented with a kinetic study of the synergistic combinations. Fifteen synergistic combinations were characterized for nine of the tested bacteria. CIN demonstrated effectiveness in reducing the MIC of chloramphenicol, streptomycin, amoxicillin, and erythromycin (94–98%) when tested on Serratia marcescens, Staphylococcus aureus, Pasteurella aerogenes, and Salmonella enterica, respectively. The kinetic study revealed that when the substances were tested alone at the MIC concentration observed in the synergistic combination, bacterial growth was not inhibited. However, when CIN and the ABX, for which synergy was observed, were tested simultaneously in combination at these same concentrations, the bacterial growth inhibition was complete. This demonstrates the highly potent in vitro synergistic activity of CIN when combined with commercial ABXs. This finding could be particularly beneficial in livestock farming, as this sector witnesses the highest quantities of antimicrobial usage, contributing significantly to antimicrobial resistance issues. Further research focused on this natural compound is thus warranted for this reason.
000129921 536__ $$9info:eu-repo/grantAgreement/ES/DGA/T67-23R
000129921 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000129921 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000129921 700__ $$aPino-Otín María Rosa
000129921 700__ $$aBallestero, Diego
000129921 700__ $$aLorca, Guillermo
000129921 700__ $$0(orcid)0000-0001-8511-5218$$aTerrado, Eva María$$uUniversidad de Zaragoza
000129921 700__ $$aLanga, Elisa
000129921 7102_ $$14013$$2205$$aUniversidad de Zaragoza$$bDpto. Didácticas Específicas$$cÁrea Didáctica Ciencias Exper.
000129921 773__ $$g13, 2 (2024), 192 [21 pp.]$$tPlants$$x2223-7747
000129921 8564_ $$s584085$$uhttps://zaguan.unizar.es/record/129921/files/texto_completo.pdf$$yVersión publicada
000129921 8564_ $$s2617526$$uhttps://zaguan.unizar.es/record/129921/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000129921 909CO $$ooai:zaguan.unizar.es:129921$$particulos$$pdriver
000129921 951__ $$a2024-07-05-12:51:22
000129921 980__ $$aARTICLE