129988 20240118092026.0 doi 10.1016/j.chroma.2007.01.138 sideral 65550 ART-2007-65550 eng Mateos, E. Universidad de Zaragoza Coralyne Cation, a Fluorescent Probe for General Detection in Planar Chromatography 2007 A large number of analytes, including non-fluorescent ones, can be sensitively detected by fluorescence scanning densitometry using silica gel HPTLC plates impregnated with a solution of coralyne cation. This is carried out by the variation, increase or decrease, that the corresponding analyte induces on native coralyne emission at a given excitation wavelength. A similar phenomenon was previously described for berberine cation, and Reichardt's dye probes. However, the sensitivity of coralyne in HPTLC detection of non-fluorescent, structurally different analytes (e.g., long-chain alkanes, alcohols, alkylbromides, neutral lipids) is superior to that of the above-mentioned probes. In this work, the analytical viability of this phenomenon for HPTLC detection using coralyne as a probe is explored, and fluorescent responses of a number of analytes on the coralyne system are rationalized in the light of a previously proposed model. This establishes that the resulting intensity for a probe in the presence of a given compound can be explained as a balance between radiative (contribution of non-specific interactions) and non-radiative processes (specific interactions), the latter producing fluorescence quenching. Experimental results and proposed model suggest that this phenomenon may be general for practically all kinds of analytes. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA/PM010-2006 info:eu-repo/grantAgreement/ES/MEC/CTQ2005-00227-PPQ info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.641 2007 CHEMISTRY, ANALYTICAL 5 / 70 = 0.071 2007 Q1 T1 BIOCHEMICAL RESEARCH METHODS 15 / 59 = 0.254 2007 Q2 T1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Cebolla,V. L. Membrado,L. Vela,J. Universidad de Zaragoza (orcid)0000-0002-4887-1652 Galvez,E. M. Matt,M. Cossio,F. P. 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 2009 X Universidad de Zaragoza Dpto. Química Analítica Proy. investigación HVA 1146, 2 (2007), 251-257 J. chromatogr. A Journal of Chromatography A 0021-9673 503516 http://zaguan.unizar.es/record/129988/files/texto_completo.pdf Postprint 1068909 http://zaguan.unizar.es/record/129988/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:129988 articulos driver 2024-01-18-09:04:14 ARTICLE