000129990 001__ 129990 000129990 005__ 20240118092026.0 000129990 0247_ $$2doi$$a10.1016/j.jbiotec.2010.12.017 000129990 0248_ $$2sideral$$a72834 000129990 037__ $$aART-2011-72834 000129990 041__ $$aeng 000129990 100__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, R. 000129990 245__ $$aSelection of reference genes for gene expression studies in rats 000129990 260__ $$c2011 000129990 5060_ $$aAccess copy available to the general public$$fUnrestricted 000129990 5203_ $$aSelection of the most stable reference gene is critical for a reliable interpretation of gene expression data using RT-PCR. In order so, 17 commonly used genes were analyzed in Wistar rat duodenum, jejunum, ileum and liver following a fat gavage and at two time periods. These reference genes were also tested in liver from Zucker (fa/fa) on a long-term dietary trial. Four strategies were used to select the most suitable reference gene for each tissue: ranking according to biological coefficient of variation and further validation by statistical comparison among groups, geNorm, NormFinder and BestKeeper programs. No agreement was observed among these approaches for a particular gene, nor a common gene for all tissues. Furthermore we demonstrated that normalising using an inadequate reference conveyed into false negative and positive results. The selection of genes provided by BestKeeper resulted in more reliable results than the other statistical packages. According to this program, Tbp, Ubc, Hprt and Rn18s were the best reference genes for duodenum, jejunum, ileum and liver, respectively following a fat gavage in Wistar rats and Rn18s for liver in another rat strain on a long-term dietary intervention. Therefore, BestKeeper is highly recommendable to select the most stable gene to be used as internal standard and the selection of a specific reference expression gene requires a validation for each tissue and experimental design. 000129990 536__ $$9info:eu-repo/grantAgreement/ES/CICYT/SAF2007-60173$$9info:eu-repo/grantAgreement/ES/DGA-FSE/B69$$9info:eu-repo/grantAgreement/ES/DGA/PI025-08$$9info:eu-repo/grantAgreement/ES/DGA/PM010-2006 000129990 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000129990 590__ $$a3.045$$b2011 000129990 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b46 / 158 = 0.291$$c2011$$dQ2$$eT1 000129990 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000129990 700__ $$0(orcid)0000-0002-0108-1004$$aNavarro, M. A.$$uUniversidad de Zaragoza 000129990 700__ $$aLarraga, A. 000129990 700__ $$aStrunk, M. 000129990 700__ $$aBarranquero, C. 000129990 700__ $$aAcín, S. 000129990 700__ $$aGuzman, M. A. 000129990 700__ $$0(orcid)0000-0003-1420-5499$$aIñigo, P.$$uUniversidad de Zaragoza 000129990 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, J.$$uUniversidad de Zaragoza 000129990 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina 000129990 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000129990 773__ $$g151, 4 (2011), 325-334$$pJ. biotechnol.$$tJournal of Biotechnology$$x0168-1656 000129990 8564_ $$s517099$$uhttps://zaguan.unizar.es/record/129990/files/texto_completo.pdf$$yPostprint 000129990 8564_ $$s917871$$uhttps://zaguan.unizar.es/record/129990/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000129990 909CO $$ooai:zaguan.unizar.es:129990$$particulos$$pdriver 000129990 951__ $$a2024-01-18-09:04:21 000129990 980__ $$aARTICLE