000129992 001__ 129992 000129992 005__ 20240118092026.0 000129992 0247_ $$2doi$$a10.1016/j.jprot.2012.07.001 000129992 0248_ $$2sideral$$a79132 000129992 037__ $$aART-2012-79132 000129992 041__ $$aeng 000129992 100__ $$aRamírez-Torres, A 000129992 245__ $$aProteomics and gene expression analyses of squalene-supplemented mice identify microsomal thioredoxin domain-containing protein 5 changes associated with hepatic steatosis 000129992 260__ $$c2012 000129992 5060_ $$aAccess copy available to the general public$$fUnrestricted 000129992 5203_ $$aSqualene is an abundant hydrocarbon present in virgin olive oil. Previous studies showed that its administration decreased atherosclerosis and steatosis in male apoE-knock-out mice. To study its effects on microsomal proteins, 1 g/kg/day of squalene was administered to those mice. After 10 weeks, hepatic fat content was assessed and protein extracts of microsomal enriched fractions from control and squalene-treated animals were analyzed by 2D-DIGE. Spots exhibiting significant differences were identified by peptide fingerprinting and MSMS analysis. Squalene administration modified the expression of thirty-one proteins involved in different metabolic functions and increased the levels of those involved in vesicle transport, protein folding and redox status. Only mRNA levels of 9 genes (Arg1, Atp5b, Cat, Hyou1, Nipsnap1, Pcca, Pcx, Pyroxd2, and Txndc5) paralleled these findings. No such mRNA changes were observed in wild-type mice receiving squalene. Thioredoxin domain-containing protein 5 (TXNDC5) protein and mRNA levels were significantly associated with hepatic fat content in apoE-ko mice. These results suggest that squalene action may be executed through a complex regulation of microsomal proteins, both at the mRNA and post-transcriptional levels and the presence of apoE may change the outcome. Txndc5 reflects the anti-steatotic properties of squalene and the sensitivity to lipid accumulation. 000129992 536__ $$9info:eu-repo/grantAgreement/ES/CICYT-FEDER/SAF 2010-14958$$9info:eu-repo/grantAgreement/ES/DGA-FSE/B69$$9info:eu-repo/grantAgreement/ES/DGA/PI025-08 000129992 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000129992 590__ $$a4.088$$b2012 000129992 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b15 / 74 = 0.203$$c2012$$dQ1$$eT1 000129992 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000129992 700__ $$aBarceló-Batllori, S 000129992 700__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, R 000129992 700__ $$aNavarro, MA 000129992 700__ $$0(orcid)0000-0002-5841-0462$$aSurra, JC$$uUniversidad de Zaragoza 000129992 700__ $$0(orcid)0000-0003-1197-4276$$aArnal, C$$uUniversidad de Zaragoza 000129992 700__ $$0(orcid)0000-0001-6627-298X$$aGuillén, N$$uUniversidad de Zaragoza 000129992 700__ $$aAcín, S 000129992 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, J$$uUniversidad de Zaragoza 000129992 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000129992 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal 000129992 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000129992 7102_ $$11000$$2807$$aUniversidad de Zaragoza$$bDpto. Anat.Pat.Med.Leg.For.To.$$cArea Toxicología 000129992 773__ $$g77 (2012), 27-39$$pJ. proteomics$$tJOURNAL OF PROTEOMICS$$x1874-3919 000129992 8564_ $$s970166$$uhttps://zaguan.unizar.es/record/129992/files/texto_completo.pdf$$yPostprint 000129992 8564_ $$s1186262$$uhttps://zaguan.unizar.es/record/129992/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000129992 909CO $$ooai:zaguan.unizar.es:129992$$particulos$$pdriver 000129992 951__ $$a2024-01-18-09:04:29 000129992 980__ $$aARTICLE