doi:10.1016/j.jprot.2012.07.001engRamírez-Torres, ABarceló-Batllori, SMartínez-Beamonte, RNavarro, MASurra, JCArnal, CGuillén, NAcín, SOsada, JProteomics and gene expression analyses of squalene-supplemented mice identify microsomal thioredoxin domain-containing protein 5 changes associated with hepatic steatosisART-2012-79132Squalene is an abundant hydrocarbon present in virgin olive oil. Previous studies showed that its administration decreased atherosclerosis and steatosis in male apoE-knock-out mice. To study its effects on microsomal proteins, 1 g/kg/day of squalene was administered to those mice. After 10 weeks, hepatic fat content was assessed and protein extracts of microsomal enriched fractions from control and squalene-treated animals were analyzed by 2D-DIGE. Spots exhibiting significant differences were identified by peptide fingerprinting and MSMS analysis. Squalene administration modified the expression of thirty-one proteins involved in different metabolic functions and increased the levels of those involved in vesicle transport, protein folding and redox status. Only mRNA levels of 9 genes (Arg1, Atp5b, Cat, Hyou1, Nipsnap1, Pcca, Pcx, Pyroxd2, and Txndc5) paralleled these findings. No such mRNA changes were observed in wild-type mice receiving squalene. Thioredoxin domain-containing protein 5 (TXNDC5) protein and mRNA levels were significantly associated with hepatic fat content in apoE-ko mice. These results suggest that squalene action may be executed through a complex regulation of microsomal proteins, both at the mRNA and post-transcriptional levels and the presence of apoE may change the outcome. Txndc5 reflects the anti-steatotic properties of squalene and the sensitivity to lipid accumulation.2012http://zaguan.unizar.es/record/12999210.1016/j.jprot.2012.07.001http://zaguan.unizar.es/record/129992oai:zaguan.unizar.es:129992info:eu-repo/grantAgreement/ES/CICYT-FEDER/SAF 2010-14958info:eu-repo/grantAgreement/ES/DGA-FSE/B69info:eu-repo/grantAgreement/ES/DGA/PI025-08JOURNAL OF PROTEOMICS 77 (2012), 27-39by-nc-ndhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccess