129992 20240118092026.0 doi 10.1016/j.jprot.2012.07.001 sideral 79132 ART-2012-79132 eng Ramírez-Torres, A Proteomics and gene expression analyses of squalene-supplemented mice identify microsomal thioredoxin domain-containing protein 5 changes associated with hepatic steatosis 2012 Squalene is an abundant hydrocarbon present in virgin olive oil. Previous studies showed that its administration decreased atherosclerosis and steatosis in male apoE-knock-out mice. To study its effects on microsomal proteins, 1 g/kg/day of squalene was administered to those mice. After 10 weeks, hepatic fat content was assessed and protein extracts of microsomal enriched fractions from control and squalene-treated animals were analyzed by 2D-DIGE. Spots exhibiting significant differences were identified by peptide fingerprinting and MSMS analysis. Squalene administration modified the expression of thirty-one proteins involved in different metabolic functions and increased the levels of those involved in vesicle transport, protein folding and redox status. Only mRNA levels of 9 genes (Arg1, Atp5b, Cat, Hyou1, Nipsnap1, Pcca, Pcx, Pyroxd2, and Txndc5) paralleled these findings. No such mRNA changes were observed in wild-type mice receiving squalene. Thioredoxin domain-containing protein 5 (TXNDC5) protein and mRNA levels were significantly associated with hepatic fat content in apoE-ko mice. These results suggest that squalene action may be executed through a complex regulation of microsomal proteins, both at the mRNA and post-transcriptional levels and the presence of apoE may change the outcome. Txndc5 reflects the anti-steatotic properties of squalene and the sensitivity to lipid accumulation. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/CICYT-FEDER/SAF 2010-14958 info:eu-repo/grantAgreement/ES/DGA-FSE/B69 info:eu-repo/grantAgreement/ES/DGA/PI025-08 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 4.088 2012 BIOCHEMICAL RESEARCH METHODS 15 / 74 = 0.203 2012 Q1 T1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Barceló-Batllori, S Martínez-Beamonte, R (orcid)0000-0002-8100-5596 Navarro, MA Surra, JC Universidad de Zaragoza (orcid)0000-0002-5841-0462 Arnal, C Universidad de Zaragoza (orcid)0000-0003-1197-4276 Guillén, N Universidad de Zaragoza (orcid)0000-0001-6627-298X Acín, S Osada, J Universidad de Zaragoza (orcid)0000-0002-8251-8457 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1000 807 Universidad de Zaragoza Dpto. Anat.Pat.Med.Leg.For.To. Area Toxicología 77 (2012), 27-39 J. proteomics JOURNAL OF PROTEOMICS 1874-3919 970166 http://zaguan.unizar.es/record/129992/files/texto_completo.pdf Postprint 1186262 http://zaguan.unizar.es/record/129992/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:129992 articulos driver 2024-01-18-09:04:29 ARTICLE