000129993 001__ 129993 000129993 005__ 20240122154814.0 000129993 0247_ $$2doi$$a10.1007/s13105-019-00682-8 000129993 0248_ $$2sideral$$a112501 000129993 037__ $$aART-2019-112501 000129993 041__ $$aeng 000129993 100__ $$aFelices, María José 000129993 245__ $$aLPS-squalene interaction on d-galactose intestinal absorption 000129993 260__ $$c2019 000129993 5060_ $$aAccess copy available to the general public$$fUnrestricted 000129993 5203_ $$aThe dynamic and complex interactions between enteric pathogens and the intestinal epithelium often lead to disturbances in the intestinal barrier, altered fluid, electrolyte, and nutrient transport and can produce an inflammatory response. Lipopolysaccharide (LPS) is a complex polymer forming part of the outer membrane of Gram-negative bacteria. On the other hand, squalene is a triterpene present in high levels in the extra-virgin olive oil that has beneficial effects against several diseases and it has also anti-oxidant and anti-inflammatory properties. The aim of this work was to study whether the squalene could eliminate the LPS effect on d-galactose intestinal absorption in rabbits and Caco-2 cells. The results have shown that squalene reduced the effects of LPS on sugar absorption. High LPS doses increased d-galactose uptake through via paracellular but also decreased the active sugar transport because the SGLT1 levels were diminished. However, the endotoxin effect on the paracellular way seemed to be more important than on the transcellular route. At the same time, an increased in RELM-ß expression was observed. This event could be related to inflammation and cause a decrease in SGLT1 levels. In addition, MLCK protein is also increased by LPS which could lead to an increase in sugar transport through tight junctions. At low doses, the LPS could inhibit SGLT1 intrinsic activity. Bioinformatic studies by docking confirm the interaction between LPS-squalene as well as occur through MLCK and SGLT-1 proteins. © 2019, University of Navarra. 000129993 536__ $$9info:eu-repo/grantAgreement/ES/CIBERObn/CB06-03-1012$$9info:eu-repo/grantAgreement/ES/DGA/B16-R17$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R 000129993 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000129993 590__ $$a2.952$$b2019 000129993 591__ $$aPHYSIOLOGY$$b29 / 81 = 0.358$$c2019$$dQ2$$eT2 000129993 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b161 / 296 = 0.544$$c2019$$dQ3$$eT2 000129993 592__ $$a0.868$$b2019 000129993 593__ $$aMedicine (miscellaneous)$$c2019$$dQ1 000129993 593__ $$aPhysiology$$c2019$$dQ2 000129993 593__ $$aBiochemistry$$c2019$$dQ2 000129993 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000129993 700__ $$aEscusol, Sara 000129993 700__ $$0(orcid)0000-0002-8100-5596$$aMartinez-Beamonte, Roberto$$uUniversidad de Zaragoza 000129993 700__ $$aGascón, Sonia$$uUniversidad de Zaragoza 000129993 700__ $$0(orcid)0000-0002-8442-8041$$aBarranquero, Cristina 000129993 700__ $$aSanchez-de-Diego, Cristina 000129993 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, Jesús$$uUniversidad de Zaragoza 000129993 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi, María Jesús$$uUniversidad de Zaragoza 000129993 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología 000129993 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología 000129993 7102_ $$11009$$2X$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Técnica. Lab. y Talleres 000129993 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000129993 773__ $$g75 (2019), 329–340$$pJ. physiol. biochem.$$tJournal of Physiology and Biochemistry$$x1138-7548 000129993 8564_ $$s1401895$$uhttps://zaguan.unizar.es/record/129993/files/texto_completo.pdf$$yPostprint 000129993 8564_ $$s695950$$uhttps://zaguan.unizar.es/record/129993/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000129993 909CO $$ooai:zaguan.unizar.es:129993$$particulos$$pdriver 000129993 951__ $$a2024-01-22-15:32:34 000129993 980__ $$aARTICLE