129998 20240118092026.0 doi 10.1002/mnfr.202000354 sideral 120569 ART-2020-120569 eng Gabás-Rivera, C. (orcid)0000-0002-2348-8702 Dietary Squalene Induces Cytochromes Cyp2b10 and Cyp2c55 Independently of Sex, Dose, and Diet in Several Mouse Models 2020 Scope: To investigate the effects of squalene, the main hydrocarbon present in extra virgin olive oil, on liver transcriptome in different animal models and to test the influence of sex on this action and its relationship with hepatic lipids. Methods and Results: To this purpose, male C57BL/6J Apoe-deficient mice are fed a purified Western diet with or without squalene during 11 weeks and hepatic squalene content is assessed, so are hepatic lipids and lipid droplets. Hepatic transcriptomic changes are studied and confirmed by RT-qPCR. Dietary characteristics and influence of squalene doses are tested in Apoe-deficient on purified chow diets with or without squalene. These diets are also given to Apoa1 and wild-type mice on C57BL/6J background and to C57BL/6J xOla129 Apoe-deficient mice. Squalene supplementation increases its hepatic content without differences among sexes and hormonal status. The Cyp2b10 and Cyp2c55 gene expressions are significantly up-regulated by the squalene intake in all models, with independence of sex, sexual hormones, dietary fat content, genetic background and dose, and in Apoe-deficient mice consuming extra-virgin olive oil. Conclusion: Hepatic squalene increases the expression of these cytochromes and their changes in virgin olive oil diets may be due to their squalene content. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/CIBERObn/CB06-03-1012 info:eu-repo/grantAgreement/ES/DGA-FSE/B16-20R info:eu-repo/grantAgreement/ES/ISCIII/CB16-10-00269 info:eu-repo/grantAgreement/ES/MINECO/PID2019-104915RB-I00 info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 5.914 2020 FOOD SCIENCE & TECHNOLOGY 16 / 143 = 0.112 2020 Q1 T1 1.495 2020 Food Science 2020 Q1 Biotechnology 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Jurado-Ruiz, E. Sánchez-Ortiz, A. Romanos, E. Universidad de Zaragoza Martínez-Beamonte, R. Universidad de Zaragoza (orcid)0000-0002-8100-5596 Navarro, M.A. Universidad de Zaragoza (orcid)0000-0002-0108-1004 Surra, J.C. Universidad de Zaragoza (orcid)0000-0002-5841-0462 Arnal, C. Universidad de Zaragoza (orcid)0000-0003-1197-4276 Rodríguez-Yoldi, M.J. Universidad de Zaragoza (orcid)0000-0002-3595-7668 Andrés-Lacueva, C. Osada, J. Universidad de Zaragoza (orcid)0000-0002-8251-8457 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 64, 20 (2020), 2000354 [14 pp.] MOL NUTR FOOD RES MOLECULAR NUTRITION & FOOD RESEARCH 1613-4125 609759 http://zaguan.unizar.es/record/129998/files/texto_completo.pdf Postprint 1671655 http://zaguan.unizar.es/record/129998/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:129998 articulos driver 2024-01-18-09:04:57 ARTICLE