000130003 001__ 130003
000130003 005__ 20240118092026.0
000130003 0247_ $$2doi$$a10.1080/10826076.2020.1866600
000130003 0248_ $$2sideral$$a122449
000130003 037__ $$aART-2021-122449
000130003 041__ $$aeng
000130003 100__ $$aCebolla, V.L.
000130003 245__ $$aScanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years
000130003 260__ $$c2021
000130003 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130003 5203_ $$aThis work is a review on HPTLC contribution to lipid analysis in complex matrices, in the period from 2010 to now, lapse of time where hyphenation with other techniques, especially Mass Spectrometry, has experienced an important growth. Scanning densitometry (SD)-UV/FL of separated lipids, as the core of detection and centerpiece for hyphenation, and its coupling with MS using soft ionization techniques (ESI, APCI, MALDI, DESI and others), are the central axis of this work. The occasional intercalation in this coupling of an on-plate biological assay for effect-direct analysis (EDA) of lipids, as well as the combination of SD with radio-densitometry (RD) using isotopically labeled-lipids are also covered topics. The described techniques make possible to develop strategies for obtaining qualitative and different levels of quantitative information, including untargeted lipids species. HPTLC of lipids has been used for: comparative purposes; fingerprinting; semi-quantitative determination, identification of species; or quantitative determination of a given individual lipid. Goals in molecular biology and biochemistry-related samples have been: preparative isolations; control of purity; verification of metabolic products; on-plate biological assays; profiles in cells; analysis of products from the cellular metabolism; measurement of enzymatic activities; monitoring lipid transport across membranes or at biological interfaces; or monitoring lipid dynamics. Abbreviations: AcH: acetic acid; AMD: Automated Multiple Development; APCI: Atmospheric Pressure Chemical Ionization; Cer: Ceramides; Chol: Cholesterol; CholE: Cholesteryl esters: CholS: Cholesteryl sulfate; DCM: Dichloromethane; DESI: Desorption Electrospray Ionization; DG: Diacylglycerides; E1: estrone; E2: 17-ß estradiol; E3: estriol; EE2: 17-a ethynylestradiol; EDA: Effect-Direct Analysis; ESI: Electrospray Ionization; EtOH: ethanol; FA: Fatty Acids; FAME: Fatty acid-methyl esters; FFA: Free Fatty Acids; GalCer: Galactosyl-Ceramides; Gb3: Globotriaosylceramides; Gb4: Globotetraosylceramides; GC: Gas Chromatography; GL: Glyco-lipids; GlcCer: Glucosyl-Ceramides; GSL: Glycosphingolipids; HPTLC: High-Performance Thin-Layer Chromatography; IMS: Ion-Mobility Separation; LacCer: Lactosyl-Ceramides; LC: Liquid Chromatography; LESA: Liquid Extraction Surface Analysis; LPC: Lyso-Phosphatidylcholines; LPG: Lyso-Phosphatidylglycerols; MALDI: Matrix Assisted Laser Desorption Ionization; m.d.: Migration distance; MeOH: Methanol; MG: Monoacylglycerides; NL: Neutral Lipids; PA: Phosphatidic Acids; PC: Phosphatidylcholines; PE: Phosphatidylethanolamines; PG: Phosphatidylglycerols; PI: Phosphatidylinositols; PL: Glycerophospholipids; PMA: Phosphomolybdic acid; PS: Phosphatidylserines; SD: Scanning Densitometry; SL: Sphingolipids; SM: Sphingomyelins; SQ: Semi-quantitative; Sq: squalene; SterylE: Steryl-esters; TG: Triacylglycerides.
000130003 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B25-20R
000130003 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000130003 590__ $$a1.467$$b2021
000130003 591__ $$aCHEMISTRY, ANALYTICAL$$b76 / 87 = 0.874$$c2021$$dQ4$$eT3
000130003 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b77 / 79 = 0.975$$c2021$$dQ4$$eT3
000130003 592__ $$a0.265$$b2021
000130003 593__ $$aAnalytical Chemistry$$c2021$$dQ3
000130003 593__ $$aPharmaceutical Science$$c2021$$dQ3
000130003 593__ $$aClinical Biochemistry$$c2021$$dQ3
000130003 594__ $$a2.3$$b2021
000130003 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000130003 700__ $$aJarne, C.
000130003 700__ $$0(orcid)0000-0002-4887-1652$$aVela, J.$$uUniversidad de Zaragoza
000130003 700__ $$0(orcid)0000-0003-2607-7834$$aGarriga, R.$$uUniversidad de Zaragoza
000130003 700__ $$aMembrado, L.
000130003 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, J.$$uUniversidad de Zaragoza
000130003 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000130003 7102_ $$12012$$2755$$aUniversidad de Zaragoza$$bDpto. Química Física$$cÁrea Química Física
000130003 773__ $$g44, 3-4 (2021), 148-170$$pJ. liq. chromatogr. relat. technol.$$tJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES$$x1082-6076
000130003 8564_ $$s715732$$uhttps://zaguan.unizar.es/record/130003/files/texto_completo.pdf$$yPostprint
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000130003 951__ $$a2024-01-18-09:05:32
000130003 980__ $$aARTICLE