130005 20240319080955.0 doi 10.1007/s00764-022-00171-7 sideral 129911 ART-2022-129911 eng Cebolla, Vicente L. Lipidomic studies based on high-performance thin-layer chromatography 2022 Access copy available to the general public Unrestricted The last triennium has shown a significant contribution of high-performance thin-layer chromatography (HPTLC) to lipidomics. HPTLC separation in combination with radio- and/or ultraviolet-fluorescence (UV-FL) densitometry was the technique of choice for tracking the transport of phospholipids among different cellular compartments in a number of biological systems, e.g., Gram-negative bacteria, yeast membranes, endoplasmic reticulum/mitochondrial membrane interface, and also to monitor the lipid transfer activity of a protein. Likewise, a significant number of HPTLC methods were developed to determine variations in the content of different lipid classes and subclasses after using genetic knockouts of cells. Radiolabeled control cells and cells whose genes were deleted using genomic editing allowed to study by HPTLC the effect of the disruption of the related proteins on the corresponding biosynthetic pathways. As well, direct interface-based coupling of HPTLC to mass spectrometry (MS) using mostly electrospray ionization(ESI) and a variety of mass analyzers, has gained new momentum in this period. Obtaining a large amount of information online in a very short time from complex biological samples, and the structural identification of target and non-target, unknown lipids, make this technique a useful tool for lipidomics. Quantitative issues related to HPTLC-MS are also discussed in this work. info:eu-repo/grantAgreement/ES/CSIC/2021-80E076 info:eu-repo/grantAgreement/ES/DGA/E25-20R info:eu-repo/grantAgreement/ES/ISCIII-AES/PI21-00036 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 1.6 2022 CHEMISTRY, ANALYTICAL 70 / 86 = 0.814 2022 Q4 T3 0.268 2022 Analytical Chemistry 2022 Q3 Clinical Biochemistry 2022 Q4 Biochemistry 2022 Q4 1.9 2022 info:eu-repo/semantics/review info:eu-repo/semantics/acceptedVersion Jarne, Carmen Membrado, Luis Escuin, Jose M. (orcid)0000-0002-5645-6723 Vela, Jesús Universidad de Zaragoza (orcid)0000-0002-4887-1652 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 35 (2022), 229-241 JPC, J. planar chromatogr. mod. TLC JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC 0933-4173 2371190 http://zaguan.unizar.es/record/130005/files/texto_completo.pdf Postprint 2118675 http://zaguan.unizar.es/record/130005/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:130005 articulos driver 2024-03-18-13:34:37 ARTICLE