000130010 001__ 130010
000130010 005__ 20241125101133.0
000130010 0247_ $$2doi$$a10.3389/fnut.2023.1287312
000130010 0248_ $$2sideral$$a136323
000130010 037__ $$aART-2023-136323
000130010 041__ $$aeng
000130010 100__ $$aDíez-Sainz, Ester
000130010 245__ $$amiR482f and miR482c-5p from edible plant-derived foods inhibit the expression of pro-inflammatory genes in human THP-1 macrophages
000130010 260__ $$c2023
000130010 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130010 5203_ $$aEdible plants can exert anti-inflammatory activities in humans, being potentially useful in the treatment of inflammatory diseases. Plant-derived microRNAs have emerged as cross-kingdom gene expression regulators and could act as bioactive molecules involved in the beneficial effects of some edible plants. We investigated the role of edible plant-derived microRNAs in the modulation of pro-inflammatory human genes.MethodsMicroRNAs from plant-derived foods were identified by next-generation sequencing. MicroRNAs with inflammatory putative targets were selected, after performing in silico analyses. The expression of candidate plant-derived miRNAs was analyzed by qPCR in edible plant-derived foods and their effects were evaluated in THP-1 monocytes differentiated to macrophages. The bioavailability of candidate plant miRNAs in humans was evaluated in feces and serum samples by qPCR.ResultsmiR482f and miR482c-5p are present in several edible plant-derived foods, such as fruits, vegetables, and cooked legumes and cereals, and fats and oils. Transfections with miR482f and miR482c-5p mimics decreased the gene expression of CLEC7A and NFAM1, and TRL6, respectively, in human THP-1 monocytes differentiated to macrophages, which had an impact on gene expression profile of inflammatory biomarkers. Both microRNAs (miR482f and miR482c-5p) resisted degradation during digestion and were detected in human feces, although not in serum.ConclusionOur findings suggest that miR482f and miR482c-5p can promote an anti-inflammatory gene expression profile in human macrophages in vitro and their bioavailability in humans can be achieved through diet, but eventually restricted at the gut level.
000130010 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/CB12-03-30002$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/BFU2015-65937-R$$9info:eu-repo/grantAgreement/ES/MINECO/PID2022-141766OB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/RTI2018-102205-B-I00
000130010 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000130010 590__ $$a4.0$$b2023
000130010 592__ $$a0.828$$b2023
000130010 591__ $$aNUTRITION & DIETETICS$$b31 / 114 = 0.272$$c2023$$dQ2$$eT1
000130010 593__ $$aFood Science$$c2023$$dQ1
000130010 593__ $$aNutrition and Dietetics$$c2023$$dQ2
000130010 593__ $$aEndocrinology, Diabetes and Metabolism$$c2023$$dQ2
000130010 594__ $$a5.2$$b2023
000130010 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130010 700__ $$0(orcid)0000-0002-1033-6152$$aLorente-Cebrián, Silvia$$uUniversidad de Zaragoza
000130010 700__ $$aAranaz, Paula
000130010 700__ $$aAmri, Ez-Zoubir
000130010 700__ $$aRiezu-Boj, José I.
000130010 700__ $$aMilagro, Fermín I.
000130010 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000130010 773__ $$g10 (2023), 1287312 [15 pp.]$$pFront. Nutr.$$tFrontiers in Nutrition$$x2296-861X
000130010 8564_ $$s1987900$$uhttps://zaguan.unizar.es/record/130010/files/texto_completo.pdf$$yVersión publicada
000130010 8564_ $$s2064767$$uhttps://zaguan.unizar.es/record/130010/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130010 909CO $$ooai:zaguan.unizar.es:130010$$particulos$$pdriver
000130010 951__ $$a2024-11-22-11:59:45
000130010 980__ $$aARTICLE