130010 20241125101133.0 doi 10.3389/fnut.2023.1287312 sideral 136323 ART-2023-136323 eng Díez-Sainz, Ester miR482f and miR482c-5p from edible plant-derived foods inhibit the expression of pro-inflammatory genes in human THP-1 macrophages 2023 Access copy available to the general public Unrestricted Edible plants can exert anti-inflammatory activities in humans, being potentially useful in the treatment of inflammatory diseases. Plant-derived microRNAs have emerged as cross-kingdom gene expression regulators and could act as bioactive molecules involved in the beneficial effects of some edible plants. We investigated the role of edible plant-derived microRNAs in the modulation of pro-inflammatory human genes.MethodsMicroRNAs from plant-derived foods were identified by next-generation sequencing. MicroRNAs with inflammatory putative targets were selected, after performing in silico analyses. The expression of candidate plant-derived miRNAs was analyzed by qPCR in edible plant-derived foods and their effects were evaluated in THP-1 monocytes differentiated to macrophages. The bioavailability of candidate plant miRNAs in humans was evaluated in feces and serum samples by qPCR.ResultsmiR482f and miR482c-5p are present in several edible plant-derived foods, such as fruits, vegetables, and cooked legumes and cereals, and fats and oils. Transfections with miR482f and miR482c-5p mimics decreased the gene expression of CLEC7A and NFAM1, and TRL6, respectively, in human THP-1 monocytes differentiated to macrophages, which had an impact on gene expression profile of inflammatory biomarkers. Both microRNAs (miR482f and miR482c-5p) resisted degradation during digestion and were detected in human feces, although not in serum.ConclusionOur findings suggest that miR482f and miR482c-5p can promote an anti-inflammatory gene expression profile in human macrophages in vitro and their bioavailability in humans can be achieved through diet, but eventually restricted at the gut level. info:eu-repo/grantAgreement/ES/ISCIII/CB12-03-30002 info:eu-repo/grantAgreement/ES/MINECO-FEDER/BFU2015-65937-R info:eu-repo/grantAgreement/ES/MINECO/PID2022-141766OB-I00 info:eu-repo/grantAgreement/ES/MINECO/RTI2018-102205-B-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.0 2023 NUTRITION & DIETETICS 31 / 114 = 0.272 2023 Q2 T1 0.828 2023 Food Science 2023 Q1 Nutrition and Dietetics 2023 Q2 Endocrinology, Diabetes and Metabolism 2023 Q2 5.2 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Lorente-Cebrián, Silvia Universidad de Zaragoza (orcid)0000-0002-1033-6152 Aranaz, Paula Amri, Ez-Zoubir Riezu-Boj, José I. Milagro, Fermín I. 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 10 (2023), 1287312 [15 pp.] Front. Nutr. Frontiers in Nutrition 2296-861X 1987900 http://zaguan.unizar.es/record/130010/files/texto_completo.pdf Versión publicada 2064767 http://zaguan.unizar.es/record/130010/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:130010 articulos driver 2024-11-22-11:59:45 ARTICLE