130041 20240731103355.0 doi 10.1093/function/zqad063 sideral 136356 ART-2023-136356 eng Lucea, Susana Intestinal and renal adaptations to changes of dietary phosphate concentrations in rat 2023 Access copy available to the general public Unrestricted We have studied the role of the intestine, kidney, and several hormones when adapting to changes in dietary P concentration. Normal and parathyroidectomized (PTX) rats were fed pH-matched diets containing 0.1%, 0.6%, and 1.2% P concentrations. 32Pi uptake was determined in the jejunum and kidney cortex brush border membrane vesicles. Several hormone and ion concentrations were determined in the blood and urine of rats. Both jejunum and kidney cortex Pi transport was regulated with 5 d of chronic feeding of P diets in normal rats. Acute adaptation was determined by switching foods on day 6, which was only clearly observed in the kidney cortex of normal rats, with more statistical variability in the jejunum. However, no paradoxical increase of Pi uptake in the jejunum was reproduced after the acute switch to the 1.2% P diet. Pi uptake in the jejunum was parathyroid hormone (PTH)-independent, but in the kidney, the chronic adaptation was reduced, and no acute dietary adaptations were observed. The NaPi2a protein was more abundant in the PTX than the sham kidneys, but contrary to the modest or absent changes in Pi uptake adaptation, the transporter was similarly regulated by dietary P, as in the sham rats. PTH and fibroblast growth factor 23 (FGF23) were the only hormones regulated by all diet changes, even in fasting animals, which exhibited regulated Pi transport despite similar phosphatemia. Evidence of Pi appetite effects was also observed. In brief, our results show new characteristics of Pi adaptations, including a lack of correlation between Pi transport, NaPi2a expression, and PTH/FGF23 concentrations. info:eu-repo/grantAgreement/ES/MICINN/PID2021-127818OB-I00 info:eu-repo/grantAgreement/ES/MINECO-FEDER/PGC2018-098635-B-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 0.987 2023 Cancer Research 2023 Q2 Physiology 2023 Q2 Molecular Medicine 2023 Q2 Cell Biology 2023 Q2 5.7 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Chopo-Escuin, Gema Universidad de Zaragoza (orcid)0000-0003-4258-3462 Guillén, Natalia Universidad de Zaragoza (orcid)0000-0001-6627-298X Sosa, Cecilia Universidad de Zaragoza (orcid)0000-0003-2907-0427 Sorribas, Víctor Universidad de Zaragoza (orcid)0000-0003-3457-323X 1002 807 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Toxicología 5, 1 (2023), [15 pp.] Function (Oxford) 2101521 http://zaguan.unizar.es/record/130041/files/texto_completo.pdf Versión publicada 2139602 http://zaguan.unizar.es/record/130041/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:130041 articulos driver 2024-07-31-09:56:27 ARTICLE