000130220 001__ 130220
000130220 005__ 20241220120715.0
000130220 0247_ $$2doi$$a10.1080/00218839.2023.2231673
000130220 0248_ $$2sideral$$a135172
000130220 037__ $$aART-2023-135172
000130220 041__ $$aeng
000130220 100__ $$aDivasón, José
000130220 245__ $$aIn vitro maintenance of drones and development of a new software for sperm quality analysis facilitate the study of honey bee reproductive quality
000130220 260__ $$c2023
000130220 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130220 5203_ $$aThis study aimed to develop a laboratory method that allows the in vitro maintenance of honey bee drones for several days while preserving their reproductive capacity and to create a new open-source software for the automatic analysis of their sperm quality. Three experiments were performed. The first experiment was designed to validate the new open-source software named CASABee for sperm quality assessment specifically designed for the honey bee. The software was able to identify motile and static spermatozoa with high precision. Results showed a high correlation between the results of sperm quality obtained both manually and by the CASABee system (0.95 and 0.96 for sperm motility and concentration, respectively, p < 0.001). In the second and third experiments, the effect of in vitro maintenance of drones without attendant workers for four days on their ejaculatory capacity and sperm quality, respectively, was evaluated. Survival rate was 98.68%, 89.48%, 75.93%, and 60.97% on average on days 1, 2, 3, and 4 after capturing, respectively. A high proportion of the drones (80.37% on average) were able to ejaculate providing semen, and there were no significant differences in the ejaculatory capacity and sperm quality of drones on the different days of in vitro maintenance, except for sperm viability, which decreased slightly on day 4 (71.71% vs 82.8% on day 0, p < 0.05). It was concluded that the new CASABee system and the method for laboratory maintenance of honey bee drones facilitate the study of reproduction in this species.
000130220 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2020-112673RB-I00$$9info:eu-repo/grantAgreement/ES/AEI/PID2020-116641GB-I00$$9info:eu-repo/grantAgreement/ES/DGA-FSE/A07-17R
000130220 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000130220 590__ $$a1.4$$b2023
000130220 592__ $$a0.449$$b2023
000130220 591__ $$aENTOMOLOGY$$b46 / 109 = 0.422$$c2023$$dQ2$$eT2
000130220 593__ $$aInsect Science$$c2023$$dQ2
000130220 594__ $$a4.8$$b2023
000130220 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000130220 700__ $$aRomero, Ana
000130220 700__ $$aSilvestre, Miguel Á
000130220 700__ $$0(orcid)0000-0001-8991-325X$$aSantolaria, Pilar$$uUniversidad de Zaragoza
000130220 700__ $$0(orcid)0000-0001-5316-1703$$aYániz, Jesús L.$$uUniversidad de Zaragoza
000130220 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000130220 773__ $$g63, 5 (2023), 1088-1095$$pJ. Apic. Res.$$tJOURNAL OF APICULTURAL RESEARCH$$x0021-8839
000130220 8564_ $$s104398$$uhttps://zaguan.unizar.es/record/130220/files/texto_completo.pdf$$yPostprint$$zinfo:eu-repo/date/embargoEnd/2024-07-10
000130220 8564_ $$s892666$$uhttps://zaguan.unizar.es/record/130220/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint$$zinfo:eu-repo/date/embargoEnd/2024-07-10
000130220 909CO $$ooai:zaguan.unizar.es:130220$$particulos$$pdriver
000130220 951__ $$a2024-12-20-12:05:29
000130220 980__ $$aARTICLE