130243 20240123131022.0 doi 10.1016/j.transproceed.2012.05.006 sideral 78271 ART-2012-78271 eng Gonzalo, M. A. Analysis of tissue bioimpedance as a measurement of liver steatosis: Experimental model in large animals 2012 Background Electrical bioimpedance (BI) has been used to indirectly measure steatosis. This method has not yet been established in the clinics thus experimental studies are needed in big animals. We assessed BI to measure liver steatosis in porcine animals. Methods Twelve large-white × Landrace pigs weighing 35 kg were allocated to a study (n = 9) and a control group (n = 3). A special diet was used to promote steatosis among the study group: methionine deficient and choline-restricted diet that contains supplements of cholesterol, collate and excess of saturated fat. Control group animals were fed a normal diet. A new tetrapolar electrode model was used for BI measurement, which were performed during open laparotomy by inserting a probe into one of the lobes. Measurements were done in the third and fourth segments of the pig liver, placing the probe either on the surface or inserted into the parenchyma of the liver. Open biopsies were obtained at the end of the measurements. Histological samples were processed and stained with hematoxylin-eosin to estimate macrosteatosis. Pearson correlation coefficient between BI and percentage steatosis were calculated at different frequencies. Results After 4 months of the special diet all the animals in the study group developed steatosis (90% to 20%), whereas none of the control group was affected. Pearson correlation coefficients between BI and percentage of steatosis were significant (0.877–0.878) with the best correlations obtained with a probe placed on the fourth segment of the liver surface and the best frequency to perform the measurements being 50 and 75 kHz. Conclusions BI is an accurate, fast method for steatosis measurements, that is easier and cheaper than either open or needle biopsy. info:eu-repo/semantics/closedAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 0.952 2012 SURGERY 130 / 198 = 0.657 2012 Q3 T2 TRANSPLANTATION 22 / 26 = 0.846 2012 Q4 T3 IMMUNOLOGY 124 / 136 = 0.912 2012 Q4 T3 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Martínez-Beamonte, R. (orcid)0000-0002-8100-5596 Palacios,P. Marí­n, J. Castiella, T. Universidad de Zaragoza (orcid)0000-0001-7453-2470 Surra, J. Universidad de Zaragoza (orcid)0000-0002-5841-0462 Burdí­o, F. Sousa, R. Universidad de Zaragoza (orcid)0000-0002-8102-5730 Güemes, A. Universidad de Zaragoza (orcid)0000-0002-4437-2581 Osada, J. Universidad de Zaragoza (orcid)0000-0002-8251-8457 Garcí­a-Gil, A. 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 1004 090 Universidad de Zaragoza Dpto. Cirugía,Ginecol.Obstetr. Área Cirugía 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1000 020 Universidad de Zaragoza Dpto. Anat.Pat.Med.Leg.For.To. Area Anatomía Patológica 44, 6 (2012), 1579-1583 Transplant. proc. Transplantation Proceedings 0041-1345 1747042 http://zaguan.unizar.es/record/130243/files/texto_completo.pdf Postprint 2498726 http://zaguan.unizar.es/record/130243/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:130243 articulos driver 2024-01-23-12:42:55 ARTICLE