000130246 001__ 130246
000130246 005__ 20240123131022.0
000130246 0247_ $$2doi$$a10.1007/s00248-016-0809-6
000130246 0248_ $$2sideral$$a96144
000130246 037__ $$aART-2016-96144
000130246 041__ $$aeng
000130246 100__ $$0(orcid)0000-0002-5797-3909$$aLatorre, E.
000130246 245__ $$aListeria monocytogenes Inhibits Serotonin Transporter in Human Intestinal Caco-2 Cells
000130246 260__ $$c2016
000130246 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130246 5203_ $$aListeria monocytogenes is a Gram-positive bacterium that can cause a serious infection. Intestinal microorganisms have been demonstrated to contribute to intestinal physiology not only through immunological responses but also by modulating the intestinal serotonergic system. Serotonin (5-HT) is a neuromodulator that is synthesized in the intestinal epithelium and regulates the whole intestinal physiology. The serotonin transporter (SERT), located in enterocytes, controls intestinal 5-HT availability and therefore serotonin’s effects. Infections caused by L. monocytogenes are well described as being due to the invasion of intestinal epithelial cells; however, the effect of L. monocytogenes on the intestinal epithelium remains unknown. The main aim of this work, therefore, was to study the effect of L. monocytogenes on SERT. Caco2/TC7 cell line was used as an enterocyte-like in vitro model, and SERT functional and molecular expression assays were performed. Our results demonstrate that living L. monocytogenes inhibits serotonin uptake by reducing SERT expression at the brush border membrane. However, neither inactivated L. monocytogenes nor soluble metabolites were able to affect SERT. The results also demonstrate that L. monocytogenes yields TLR2 and TLR10 transcriptional changes in intestinal epithelial cells and suggest that TLR10 is potentially involved in the inhibitory effect observed on SERT. Therefore, L. monocytogenes, through TLR10-mediated SERT inhibition, may induce increased intestinal serotonin availability and potentially contributing to intestinal physiological changes and the initiation of the inflammatory response.
000130246 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000130246 590__ $$a3.63$$b2016
000130246 591__ $$aECOLOGY$$b37 / 153 = 0.242$$c2016$$dQ1$$eT1
000130246 591__ $$aMARINE & FRESHWATER BIOLOGY$$b4 / 105 = 0.038$$c2016$$dQ1$$eT1
000130246 591__ $$aMICROBIOLOGY$$b34 / 122 = 0.279$$c2016$$dQ2$$eT1
000130246 592__ $$a1.325$$b2016
000130246 593__ $$aEcology$$c2016$$dQ1
000130246 593__ $$aSoil Science$$c2016$$dQ1
000130246 593__ $$aEcology, Evolution, Behavior and Systematics$$c2016$$dQ1
000130246 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130246 700__ $$aPradilla, A.
000130246 700__ $$aChueca, B.
000130246 700__ $$aPagán, R.
000130246 700__ $$0(orcid)0000-0001-5573-6144$$aLayunta, E.$$uUniversidad de Zaragoza
000130246 700__ $$0(orcid)0000-0002-9935-927X$$aAlcalde, A. I.
000130246 700__ $$0(orcid)0000-0003-4758-3998$$aMesonero, J. E.$$uUniversidad de Zaragoza
000130246 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000130246 773__ $$g72 , 3 (2016), 730–739$$pMicrob. ecol.$$tMICROBIAL ECOLOGY$$x0095-3628
000130246 8564_ $$s817328$$uhttps://zaguan.unizar.es/record/130246/files/texto_completo.pdf$$yVersión publicada
000130246 8564_ $$s2254343$$uhttps://zaguan.unizar.es/record/130246/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130246 909CO $$ooai:zaguan.unizar.es:130246$$particulos$$pdriver
000130246 951__ $$a2024-01-23-12:43:11
000130246 980__ $$aARTICLE