000130256 001__ 130256
000130256 005__ 20240124152523.0
000130256 0247_ $$2doi$$a10.33594/000000058
000130256 0248_ $$2sideral$$a136546
000130256 037__ $$aART-2019-136546
000130256 041__ $$aeng
000130256 100__ $$0(orcid)0000-0001-5573-6144$$aLayunta Hernández, Elena$$uUniversidad de Zaragoza
000130256 245__ $$aIntestinal Response to Acute Intragastric and Intravenous Administration of Phosphate in Rats
000130256 260__ $$c2019
000130256 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130256 5203_ $$aBackground/Aims: Phosphate (Pi) homeostasis is controlled by the intestine and kidneys whose capacities to transport Pi are under endocrine control. Several studies point to intestinal absorption as a therapeutic target to modulate Pi homeostasis. The small intestine is responsible for almost all Pi absorption in the gut, a process involving Na+-dependent and independent mechanisms. Three Na+-dependent Pi cotransporters have been described in the gastrointestinal tract: NaPi-IIb (a SLC34 member) and Pit-1 and Pit-2 (SLC20 transporters). We recently analysed the acute hormonal and renal response to intragastric (i.g) and intravenous (i.v) Pi-loading. This study demonstrated that the kidney quickly adapts to Pi-loading, with changes manifesting earlier in the i.v than i.g intervention. The aim of this work was to extend the previous studies in order to investigate the acute adaptation of intestinal transport of Pi and expression of intestinal Na+/Pi-cotransporters in response to acute Pi-loading.
Methods:
Duodenal and jejunal mucosa was collected 40 minutes and/or 4 hours after administration (i.g and i.v) of either NaCl or Pi to anaesthetized rats. Uptakes of Pi and protein expression of Na+/Pi cotransporters were measured in brush border membrane vesicles (BBMV); the cotransporters’ mRNA abundance was quantified by real-time PCR in total RNA extracted
from whole mucosa.
Results: Pi-loading did not modify transport of Pi in duodenal and jejunal BBMV 4 hours after treatment. Administration of Pi did not alter either the intestinal expression of NaPi-IIb and Pit-2 mRNAs, whereas Pit-1 mRNA expression was only regulated (diminished) in duodenum collected 4 hours after i.g Pi-loading. NaPi-IIb protein expression was decreased
in duodenum 4 hours upon i.v Pi infusion, whereas the duodenal and jejunal abundance of the cotransporter was unaffected by i.g administration of Pi. Conclusion: Together, these data suggest that the intestine responds acutely to Pi-loading, though this response seems slower than the renal adaptation.
000130256 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000130256 592__ $$a1.303$$b2019
000130256 593__ $$aPhysiology$$c2019$$dQ1
000130256 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130256 700__ $$aPastor Arroyo, Eva Maria
000130256 700__ $$aKägi, Larissa
000130256 700__ $$aThomas, Linto
000130256 700__ $$aLevi, Moshe
000130256 700__ $$aHernando, Nati
000130256 700__ $$aWagner, Carsten A
000130256 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000130256 773__ $$g52, 4 (2019), 838-849$$pCell. physiol. biochem.$$tCellular physiology and biochemistry$$x1015-8987
000130256 8564_ $$s1246461$$uhttps://zaguan.unizar.es/record/130256/files/texto_completo.pdf$$yVersión publicada
000130256 8564_ $$s1950606$$uhttps://zaguan.unizar.es/record/130256/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130256 909CO $$ooai:zaguan.unizar.es:130256$$particulos$$pdriver
000130256 951__ $$a2024-01-24-15:20:59
000130256 980__ $$aARTICLE