130263 20240124152850.0 doi 10.1089/bio.2012.0015 sideral 92401 ART-2012-92401 eng Solanas, E Incubation with dimethyl sulfoxide prior to cryopreservation improves functionality of thawed human primary hepatocytes 2012 Background: Efficient cryopreservation of human hepatocytes is essential for their use in cell therapy. This study investigated the effects of adding melatonin and/or dimethyl sulfoxide (DMSO) to pre-incubation and/or freezing solutions on the viability and function of thawed human hepatocytes. Methods: Isolated human hepatocytes were pre-incubated for 90 min at 4°C in Williams' Medium E (WEM), WEM containing 5 mM melatonin dissolved in DMSO, or WEM containing the equivalent amount of DMSO (1%). The hepatocytes were frozen in University of Wisconsin solution (UW) and 10% DMSO, with or without 5 mM melatonin. After thawing, viability, plating efficiency, mitochondrial dehydrogenase activity (MTT), and albumin and urea production were analyzed. Results: Viability and plating efficiency were not affected by melatonin or DMSO in pre-incubation media. Unexpectedly, hepatocytes pre-incubated with DMSO had significantly higher MTT (29.7% vs. control, p<0.01), albumin (82.8% vs. control, p<0.05), and urea amounts (26.2% vs. control, p=0.06) than those incubated only with WEM. Hepatocytes pre-incubated in media containing melatonin had amounts between those of cells incubated with DMSO or only with WEM (p<0.05 for MTT and p>0.05 for albumin and urea values). Also, the addition of melatonin to the freezing media did not significantly improve any of the studied parameters (p>0.05). Discussion: Adding 1% DMSO to pre-incubation media prior to the cryopreservation of human hepatocytes preserves hepatocyte function after thawing. These findings could be considered in current hepatocyte cryopreservation protocols. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 1.5 2012 MEDICAL LABORATORY TECHNOLOGY 16 / 31 = 0.516 2012 Q3 T2 CELL BIOLOGY 157 / 183 = 0.858 2012 Q4 T3 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sostres, C Serrablo, A García-Gil, A Aranguren, F Jimenez, P Serrano, MT. Universidad de Zaragoza (orcid)0000-0002-7119-2244 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 10, 5 (2012), 446-53 BIOPRESERVATION AND BIOBANKING BIOPRESERVATION AND BIOBANKING 1947-5535 207062 http://zaguan.unizar.es/record/130263/files/texto_completo.pdf Versión publicada 2610603 http://zaguan.unizar.es/record/130263/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:130263 articulos driver 2024-01-24-14:57:54 ARTICLE