000130266 001__ 130266
000130266 005__ 20240124152850.0
000130266 0247_ $$2doi$$a10.1159/000433521
000130266 0248_ $$2sideral$$a92400
000130266 037__ $$aART-2015-92400
000130266 041__ $$aeng
000130266 100__ $$0(orcid)0000-0001-8056-5236$$aSolanas, E.$$uUniversidad de Zaragoza
000130266 245__ $$aEffect of Dimethyl Sulfoxide and Melatonin on the Isolation of Human Primary Hepatocytes
000130266 260__ $$c2015
000130266 5203_ $$aThe availability of fully functional human hepatocytes is critical for progress in human hepatocyte transplantation and the development of bioartificial livers and in vitro liver systems. However, the cell isolation process impairs the hepatocyte status and determines the number of viable cells that can be obtained. This study aimed to evaluate the effects of using dimethyl sulfoxide (DMSO) and melatonin in the human hepatocyte isolation protocol. Human hepatocytes were isolated from liver pieces resected from 10 patients undergoing partial hepatectomy. Each piece was dissected into 2 equally sized pieces and randomized, in 5 of 10 isolations, to perfusion with 1% DMSO-containing perfusion buffer or buffer also containing 5 mM melatonin using the 2-step collagenase perfusion technique (experiment 1), and in the other 5 isolations to standard perfusion or perfusion including 1% DMSO (experiment 2). Tissues perfused with DMSO yielded 70.6% more viable hepatocytes per gram of tissue (p = 0.076), with a 26.1% greater albumin production (p < 0.05) than those perfused with control buffer. Melatonin did not significantly affect (p > 0.05) any of the studied parameters, but cell viability, dehydrogenase activity, albumin production, urea secretion, and 7-ethoxycoumarin O-deethylase activity were slightly higher in cells isolated with melatonin-containing perfusion buffer compared to those isolated with DMSO. In conclusion, addition of 1% DMSO to the hepatocyte isolation protocol could improve the availability and functionality of hepatocytes for transplantation, but further studies are needed to clarify the mechanisms involved.
000130266 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130266 590__ $$a1.228$$b2015
000130266 591__ $$aANATOMY & MORPHOLOGY$$b12 / 21 = 0.571$$c2015$$dQ3$$eT2
000130266 591__ $$aDEVELOPMENTAL BIOLOGY$$b37 / 41 = 0.902$$c2015$$dQ4$$eT3
000130266 591__ $$aCELL BIOLOGY$$b170 / 187 = 0.909$$c2015$$dQ4$$eT3
000130266 592__ $$a0.672$$b2015
000130266 593__ $$aHistology$$c2015$$dQ2
000130266 593__ $$aAnatomy$$c2015$$dQ2
000130266 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130266 700__ $$aSostres, C.
000130266 700__ $$0(orcid)0000-0003-4712-9891$$aSerrablo, A.$$uUniversidad de Zaragoza
000130266 700__ $$0(orcid)0000-0002-8275-7191$$aGarcía-Gil, A.$$uUniversidad de Zaragoza
000130266 700__ $$0(orcid)0000-0001-9507-6478$$aGarcía, J.J.$$uUniversidad de Zaragoza
000130266 700__ $$aAranguren, F.J.
000130266 700__ $$aJiménez, P.
000130266 700__ $$aHughes, R.D.
000130266 700__ $$0(orcid)0000-0002-7119-2244$$aSerrano, M.T.$$uUniversidad de Zaragoza
000130266 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000130266 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000130266 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000130266 7102_ $$11004$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Cirugía
000130266 773__ $$g200, 5 (2015), 316-325$$pCells tissues organs$$tCELLS TISSUES ORGANS$$x1422-6405
000130266 8564_ $$s235164$$uhttps://zaguan.unizar.es/record/130266/files/texto_completo.pdf$$yVersión publicada
000130266 8564_ $$s2114599$$uhttps://zaguan.unizar.es/record/130266/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130266 909CO $$ooai:zaguan.unizar.es:130266$$particulos$$pdriver
000130266 951__ $$a2024-01-24-14:58:14
000130266 980__ $$aARTICLE