130266 20240124152850.0 doi 10.1159/000433521 sideral 92400 ART-2015-92400 eng Solanas, E. Universidad de Zaragoza (orcid)0000-0001-8056-5236 Effect of Dimethyl Sulfoxide and Melatonin on the Isolation of Human Primary Hepatocytes 2015 The availability of fully functional human hepatocytes is critical for progress in human hepatocyte transplantation and the development of bioartificial livers and in vitro liver systems. However, the cell isolation process impairs the hepatocyte status and determines the number of viable cells that can be obtained. This study aimed to evaluate the effects of using dimethyl sulfoxide (DMSO) and melatonin in the human hepatocyte isolation protocol. Human hepatocytes were isolated from liver pieces resected from 10 patients undergoing partial hepatectomy. Each piece was dissected into 2 equally sized pieces and randomized, in 5 of 10 isolations, to perfusion with 1% DMSO-containing perfusion buffer or buffer also containing 5 mM melatonin using the 2-step collagenase perfusion technique (experiment 1), and in the other 5 isolations to standard perfusion or perfusion including 1% DMSO (experiment 2). Tissues perfused with DMSO yielded 70.6% more viable hepatocytes per gram of tissue (p = 0.076), with a 26.1% greater albumin production (p < 0.05) than those perfused with control buffer. Melatonin did not significantly affect (p > 0.05) any of the studied parameters, but cell viability, dehydrogenase activity, albumin production, urea secretion, and 7-ethoxycoumarin O-deethylase activity were slightly higher in cells isolated with melatonin-containing perfusion buffer compared to those isolated with DMSO. In conclusion, addition of 1% DMSO to the hepatocyte isolation protocol could improve the availability and functionality of hepatocytes for transplantation, but further studies are needed to clarify the mechanisms involved. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 1.228 2015 ANATOMY & MORPHOLOGY 12 / 21 = 0.571 2015 Q3 T2 DEVELOPMENTAL BIOLOGY 37 / 41 = 0.902 2015 Q4 T3 CELL BIOLOGY 170 / 187 = 0.909 2015 Q4 T3 0.672 2015 Histology 2015 Q2 Anatomy 2015 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sostres, C. Serrablo, A. Universidad de Zaragoza (orcid)0000-0003-4712-9891 García-Gil, A. Universidad de Zaragoza (orcid)0000-0002-8275-7191 García, J.J. Universidad de Zaragoza (orcid)0000-0001-9507-6478 Aranguren, F.J. Jiménez, P. Hughes, R.D. Serrano, M.T. Universidad de Zaragoza (orcid)0000-0002-7119-2244 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 1004 090 Universidad de Zaragoza Dpto. Cirugía,Ginecol.Obstetr. Área Cirugía 200, 5 (2015), 316-325 Cells tissues organs CELLS TISSUES ORGANS 1422-6405 235164 http://zaguan.unizar.es/record/130266/files/texto_completo.pdf Versión publicada 2114599 http://zaguan.unizar.es/record/130266/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:130266 articulos driver 2024-01-24-14:58:14 ARTICLE