000130273 001__ 130273
000130273 005__ 20240125154835.0
000130273 0247_ $$2doi$$a10.1097/MEG.0000000000000131
000130273 0248_ $$2sideral$$a86505
000130273 037__ $$aART-2014-86505
000130273 041__ $$aeng
000130273 100__ $$aCampillo, A.
000130273 245__ $$aAngiogenesis and proliferation markers in adjacent cirrhotic tissue could predict hepatocellular carcinoma outcome after liver transplantation
000130273 260__ $$c2014
000130273 5203_ $$aIntroduction
The current staging systems for hepatocellular carcinoma (HCC) do not sufficiently predict outcomes after liver transplantation (LT). The present study assessed whether some tissue markers related to proliferation and angiogenesis have prognostic value.
Patients and methods
The expression of CD34, vascular endothelial growth factor (VEGF), VEGFR2, VEGFR1, angiopoietin-1, angiopoietin-2, TIE2, COX-2, and proliferating cell nuclear antigen (PCNA) in tumor and adjacent cirrhotic tissue samples from 36 patients with HCC (n=10 with tumor recurrence after LT) was determined by immunochemistry. Microvessel density was assessed by CD34 staining and the PCNA labeling index calculated as the percentage of positive cells among at least 1000 hepatocyte nuclei studied in each sample using the computer program ContimUZ. VEGF, VEGFR2, VEGFR-1, angiopoietin-1, angiopoietin-2, TIE2, and COX-2 staining were evaluated by two blinded pathologists. The tumor recurrence rate was analyzed after a minimum follow-up of 36 months.
Results
A higher proliferation index in both tumor and adjacent cirrhotic tissue was related to HCC recurrence. The proliferation index in tumor tissue was also related to microvascular invasion. High expression (staining in ≥50% of hepatocytes) of COX2 [P=0.025, odds ratio (OR)=7.5, 95% confidence interval (CI) 1.3–43.4], VEGF (P=0.01, OR=12, 95% CI 1.8–80.4), and its receptor VEGFR-2 (P=0.02, OR=8.5, 95% CI 1.4–49.5) in cirrhotic liver tissue, but not tumor tissue, was related to HCC recurrence after LT.
Conclusion
A high proliferation index in tumor and cirrhotic tissue and high expression levels of some angiogenic markers in adjacent cirrhotic tissue could be predictive of tumor recurrence after LT.
000130273 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130273 590__ $$a2.253$$b2014
000130273 591__ $$aGASTROENTEROLOGY & HEPATOLOGY$$b47 / 76 = 0.618$$c2014$$dQ3$$eT2
000130273 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130273 700__ $$0(orcid)0000-0001-8056-5236$$aSolanas, E.$$uUniversidad de Zaragoza
000130273 700__ $$0(orcid)0000-0002-9617-3744$$aMorandeira, M. J.$$uUniversidad de Zaragoza
000130273 700__ $$aCastiella, T.
000130273 700__ $$aLorente, S.
000130273 700__ $$0(orcid)0000-0002-8275-7191$$aGarcia-Gil, F.$$uUniversidad de Zaragoza
000130273 700__ $$0(orcid)0000-0001-5813-3445$$aPiazuelo, E.$$uUniversidad de Zaragoza
000130273 700__ $$aDe Martino, A.
000130273 700__ $$0(orcid)0000-0002-7119-2244$$aSerrano, M. T.$$uUniversidad de Zaragoza
000130273 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000130273 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000130273 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000130273 7102_ $$11004$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Cirugía
000130273 7102_ $$11000$$2020$$aUniversidad de Zaragoza$$bDpto. Anat.Pat.Med.Leg.For.To.$$cArea Anatomía Patológica
000130273 773__ $$g26, 8 (2014), 871-879$$pEur. j. gastroenterol. hepatol.$$tEuropean Journal of Gastroenterology and Hepatology$$x0954-691X
000130273 8564_ $$s599607$$uhttps://zaguan.unizar.es/record/130273/files/texto_completo.pdf$$yVersión publicada
000130273 8564_ $$s3017722$$uhttps://zaguan.unizar.es/record/130273/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130273 909CO $$ooai:zaguan.unizar.es:130273$$particulos$$pdriver
000130273 951__ $$a2024-01-25-15:08:08
000130273 980__ $$aARTICLE