000130275 001__ 130275
000130275 005__ 20240124152850.0
000130275 0247_ $$2doi$$a10.1136/gut.50.1.13
000130275 0248_ $$2sideral$$a51932
000130275 037__ $$aART-2002-51932
000130275 041__ $$aeng
000130275 100__ $$aLorente, S.
000130275 245__ $$aHelicobacter pylori stimulates pepsinogen secretion from isolated human peptic cells.
000130275 260__ $$c2002
000130275 5203_ $$aBackground: Different acid and peptic related gastroduodenal diseases are associated with both increased gastric secretion and Helicobacter pylori infection. Patients with H pylori associated gastritis or duodenal ulcer have increased serum pepsinogen levels which decrease after eradication. The mechanisms of H pylori induced gastric mucosal damage are not completely understood.
Aim: To determine the effects of H pylori on pepsinogen secretion from isolated human peptic cells.
Methods: Dispersed human peptic cells were prepared from endoscopically obtained biopsy specimens after collagenase digestion, mechanical disruption, and density gradient centrifugation. H pylori was obtained from gastric biopsies (antrum and body), and cultured in non-selective and selective media. Isolates of H pylori were used at different concentrations (1–20×106 colony forming units (cfu)).
Results: H pylori (106–2×107 cfu) increased basal pepsinogen secretion in a concentration dependent manner. This stimulus was not observed with Escherichia coli. The increased secretion was in addition to that observed with 0.1 mM histamine and 0.1 mM dibutyryl-cyclic adenosine monophosphate. However, H pylori did not affect either carbamylcholine (0.1–10 μM) or cholecystokinin (1 μM) stimulated pepsinogen secretion. Addition of the nitric oxide synthase inhibitor Nw-monomethyl-L-arginine (1 mM) inhibited H pylori induced cGMP generation and pepsinogen secretion, which were also reduced in the absence of extracellular calcium. H pylori induced pepsinogen secretion was not affected by the absence/presence of the cagA gene.
Conclusions: H pylori increases pepsinogen secretion from human peptic cells through a calcium and nitric oxide mediated intracellular pathway. This effect is independent of the H pylori virulent cagA gene, and may be a mechanism of H pylori induced gastric mucosal damage.
000130275 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130275 590__ $$a6.323$$b2002
000130275 591__ $$aGASTROENTEROLOGY & HEPATOLOGY$$b3 / 45 = 0.067$$c2002$$dQ1$$eT1
000130275 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130275 700__ $$aDoiz, O.
000130275 700__ $$0(orcid)0000-0002-7119-2244$$aSerrano, M.T.
000130275 700__ $$0(orcid)0000-0002-2519-701X$$aCastillo, J.$$uUniversidad de Zaragoza
000130275 700__ $$0(orcid)0000-0001-5932-2889$$aLanas, A.$$uUniversidad de Zaragoza
000130275 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000130275 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000130275 773__ $$g50, 1 (2002), 13-8$$pGut$$tGUT$$x0017-5749
000130275 8564_ $$s387300$$uhttps://zaguan.unizar.es/record/130275/files/texto_completo.pdf$$yVersión publicada
000130275 8564_ $$s3146837$$uhttps://zaguan.unizar.es/record/130275/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130275 909CO $$ooai:zaguan.unizar.es:130275$$particulos$$pdriver
000130275 951__ $$a2024-01-24-14:59:03
000130275 980__ $$aARTICLE