000130331 001__ 130331 000130331 005__ 20250116144525.0 000130331 0247_ $$2doi$$a10.1039/d1ja00068c 000130331 0248_ $$2sideral$$a126254 000130331 037__ $$aART-2021-126254 000130331 041__ $$aeng 000130331 100__ $$0(orcid)0000-0002-7931-3901$$aAbad-Alvaro I. 000130331 245__ $$aAn insight into the determination of size and number concentration of silver nanoparticles in blood using single particle ICP-MS (spICP-MS): Feasibility of application to samples relevant to: In vivo toxicology studies 000130331 260__ $$c2021 000130331 5060_ $$aAccess copy available to the general public$$fUnrestricted 000130331 5203_ $$aToxicological studies concerning nanomaterials in complex biological matrices usually require a carefully designed workflow that involves handling, transportation and preparation of a large number of samples without affecting the nanoparticle (NP) characteristics, as well as measurement methods that enable their reliable characterisation. This work describes method development for the determination of number concentration and size of silver nanoparticles (AgNP) in blood for the purpose of application to the typical workflow of an in vivo toxicology assessment involving blood-containing AgNP and Ag(i) leached out from medical devices. A systematic comparison of single particle detection using millisecond versus microsecond dwell times in the presence of different ionic Ag [Ag(i)]-to-AgNP ratios by spICP-MS was undertaken to achieve sufficient selectivity for the determination of NP number concentration. This was achieved for the first time in a complex media such as 2.5% tetramethylammonium hydroxide (TMAH)/0.1% Triton X-100 (v/v) diluent, which was proven to preserve nanoparticle stability upon 8 days of storage following AgNP quantitative extraction from the blood matrix. The potential of microsecond dwell time for improved discrimination of AgNP (40 nm) from Ag(i) was demonstrated for ionic to nanoparticle ratios [Ag(i)/AgNP] of up to 106-fold. For NP sizing, a systematic study of the impact of matrix-matched ionic calibration on the derived particle size by spICP-MS is also described. Three different ionic calibration media including 1% HNO3 (v/v), 1 mM trisodium citrate and 2.5% TMAH/0.1% Triton X-100 (v/v) were tested. Student t-test evidenced statistically significant differences between the slope of the calibration curve of Ag(i) in TMAH/Triton X-100 compared to HNO3 and trisodium citrate matrices, whereas no significant differences were found between the two latter media. Moreover, a good agreement was found between the particle diameter derived from spICP-MS following ionic calibration in TMAH/Triton X-100 and the diameter obtained with transmission electron microscopy (TEM), demonstrating the importance of matrix-matched calibration for NP size determination in a complex matrix using spICP-MS. Number concentration recovery measurements on blood samples spiked with AgNP and size measurements both using spICP-MS demonstrated the feasibility of the methodology developed here for potential future application to AgNP characterisation in toxicology research. © The Royal Society of Chemistry. 000130331 536__ $$9info:eu-repo/grantAgreement/ES/MICIU/PGC2018-093753-B-I00$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-64684-P 000130331 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000130331 590__ $$a4.351$$b2021 000130331 591__ $$aSPECTROSCOPY$$b7 / 43 = 0.163$$c2021$$dQ1$$eT1 000130331 591__ $$aCHEMISTRY, ANALYTICAL$$b23 / 87 = 0.264$$c2021$$dQ2$$eT1 000130331 592__ $$a0.795$$b2021 000130331 593__ $$aSpectroscopy$$c2021$$dQ1 000130331 593__ $$aAnalytical Chemistry$$c2021$$dQ1 000130331 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000130331 700__ $$aLeite D. 000130331 700__ $$aBartczak D. 000130331 700__ $$aCuello-Nunez S. 000130331 700__ $$aGomez-Gomez B. 000130331 700__ $$aMadrid Y. 000130331 700__ $$0(orcid)0000-0002-3916-9992$$aAramendia M.$$uUniversidad de Zaragoza 000130331 700__ $$0(orcid)0000-0002-7450-8769$$aResano M.$$uUniversidad de Zaragoza 000130331 700__ $$aGoenaga-Infante H. 000130331 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica 000130331 773__ $$g36, 6 (2021), 1180-1192$$pJ. anal. at. spectrom.$$tJournal of Analytical Atomic Spectrometry$$x0267-9477 000130331 8564_ $$s1694837$$uhttps://zaguan.unizar.es/record/130331/files/texto_completo.pdf$$yPostprint 000130331 8564_ $$s1406394$$uhttps://zaguan.unizar.es/record/130331/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000130331 909CO $$ooai:zaguan.unizar.es:130331$$particulos$$pdriver 000130331 951__ $$a2025-01-16-14:42:54 000130331 980__ $$aARTICLE